Chemical Proteomics

1. Front Matter

   Pages i-xi

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. Mass Spectrometry-Based Chemoproteomic Approaches

      • Marcus Bantscheff

      Pages 3-13

   3. Chemical Proteomics in Drug Discovery

      • Gerard Drewes

      Pages 15-21

3. Small Molecules and Probe Design

   1. Front Matter

      Pages 23-23

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   2. Compound Immobilization and Drug-Affinity Chromatography

      • Uwe Rix, Manuela Gridling, Giulio Superti-Furga

      Pages 25-38

   3. Affinity-Based Chemoproteomics with Small Molecule-Peptide Conjugates

      • Chaitanya Saxena

      Pages 39-54

   4. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

      • Xia Ge, Daniel S. Sem

      Pages 55-64

   5. Probing Proteomes with Benzophenone Photoprobes

      • Akira Kawamura, Doina M. Mihai

      Pages 65-75

   6. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

      • Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, Daniel C. Liebler

      Pages 77-95

   7. Profiling of Methyltransferases and Other S-Adenosyl-l-Homocysteine-Binding Proteins by Capture Compound Mass Spectrometry

      • Thomas Lenz, Peter Poot, Elmar Weinhold, Mathias Dreger

      Pages 97-125

4. Target Discovery and Target Validation

   1. Front Matter

      Pages 127-127

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   2. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

      • Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, Steven A. Carr

      Pages 129-140

   3. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

      • Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, Marcus Bantscheff

      Pages 141-155

   4. Affinity-Based Profiling of Dehydrogenase Subproteomes

      • Xia Ge, Daniel S. Sem

      Pages 157-165

   5. Probing the Specificity of Protein–Protein Interactions by Quantitative Chemical Proteomics

      • Duangnapa Kovanich, Thin Thin Aye, Albert J. R. Heck, Arjen Scholten

      Pages 167-181

   6. Fluorescence-Based Proteasome Activity Profiling

      • Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, Celia R. Berkers

      Pages 183-204

   7. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein–Drug Interactions

      • Mathias Q. Müller, Andrea Sinz

      Pages 205-218

   8. Monitoring Ligand Modulation of Protein–Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry

      • Natalia Gasilova, Alexis Nazabal

      Pages 219-229

   9. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

      • Amy Hye Won Jeon, Gerold Schmitt-Ulms

      Pages 231-246

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

• Mass Spectrometry
• amino acids in cell culture (SILAC)
• based chemical probes
• isotope labeling
• molecule ligand
• molecules
• protein function
• quantitative immunodetection

• CellZome AG, Heidelberg, Germany

  Gerard Drewes, Marcus Bantscheff

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