Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step readily reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 794)
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Table of contents (27 protocols)
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Synthesis of Unnatural Amino Acids
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Application of Unnatural Amino Acids
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Use of Unnatural Amino Acids in Protein Synthesis
Keywords
About this book
Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.
Editors and Affiliations
Bibliographic Information
Book Title: Unnatural Amino Acids
Book Subtitle: Methods and Protocols
Editors: Loredano Pollegioni, Stefano Servi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-331-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-330-1Published: 29 September 2011
Softcover ISBN: 978-1-4939-5888-7Published: 23 August 2016
eBook ISBN: 978-1-61779-331-8Published: 30 September 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 409
Number of Illustrations: 123 b/w illustrations
Topics: Biochemistry, general, Enzymology