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  • © 2011

Neuropeptides

Methods and Protocols

  • Features contributions from scientists with direct experience in one or more of the fields of neuropeptide research
  • Include detailed protocols relevant to a wide range of scientists
  • Provides key tips and expert implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 789)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. What Are Neuropeptides?

    • J. Peter H. Burbach
    Pages 1-36
  3. Neuropeptide Localization in Nonmammalian Vertebrates

    • Paolo de Girolamo, Carla Lucini
    Pages 37-56
  4. Neuropeptide RNA Localization in Tissue Sections

    • Marc Landry, Shérine Abdel Salam, Marie Moftah
    Pages 73-87
  5. Intron-Specific Neuropeptide Probes

    • Harold Gainer, Todd A. Ponzio, Chunmei Yue, Makoto Kawasaki
    Pages 89-110
  6. Direct In Situ RT-PCR

    • Laura Lossi, Graziana Gambino, Chiara Salio, Adalberto Merighi
    Pages 111-126
  7. Laser Capture Microdissection and Quantitative-PCR Analysis

    • Sarah J. Paulsen, Leif K. Larsen
    Pages 127-135
  8. Visualization of Peptide Secretory Vesicles in Living Nerve Cells

    • Joshua J. Park, Y. Peng Loh
    Pages 137-145
  9. Analysis of Neuroimmune Interactions by an In Vitro Coculture Approach

    • Tadahide Furuno, Mamoru Nakanishi
    Pages 171-180
  10. Electrophysiology

    • Zhi-Qing David Xu
    Pages 181-189
  11. Localization of Neuropeptides by Radioimmunoassay

    • Fred Nyberg, Mathias Hallberg
    Pages 191-201
  12. Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics

    • Anne-Marie Hesse, Sega Ndiaye, Joelle Vinh
    Pages 203-221
  13. Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis

    • Ping Yin, Xiaowen Hou, Elena V. Romanova, Jonathan V. Sweedler
    Pages 223-236
  14. Suppression Subtractive Hybridization

    • Mohamed T. Ghorbel, David Murphy
    Pages 237-259
  15. Neuropeptide Microdialysis in Free-Moving Animals

    • Tetsuya Kushikata, Kazuyoshi Hirota
    Pages 261-269
  16. Antibody Microprobes for Detecting Neuropeptide Release

    • Rebecca J. Steagall, Carole A. Williams, Arthur W. Duggan
    Pages 271-285
  17. Neuropeptidases

    • Manuel Ramírez, Isabel Prieto, Inmaculada Banegas, Ana B. Segarra, Francisco Alba
    Pages 287-294
  18. Neuropeptide Autoantibodies Assay

    • Sergueï O. Fetissov
    Pages 295-302

About this book

Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy.  Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field.  The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.

Reviews

From the reviews:

“Neuropeptides: Methods and Protocols is a cutting-edge resource for any lab working in the area of neuropeptides. The overall goal of this book is to highlight a variety of important methods in neuropeptide research and provide protocols for executing those protocols. … this is a terrific contribution to neuropeptide research, a one-stop resource that will benefit all scientists interested in neuropeptides.” (Alan Saghatelian, ChemBioChem, Vol. 13 (6), April, 2012)

Editors and Affiliations

  • , Dipt. di Morfofisiologia Veterinaria, Università degli Studi di Torino, Grugliasco, Italy

    Adalberto Merighi

Bibliographic Information

  • Book Title: Neuropeptides

  • Book Subtitle: Methods and Protocols

  • Editors: Adalberto Merighi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-310-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-309-7Published: 16 September 2011

  • Softcover ISBN: 978-1-4939-6211-2Published: 30 April 2017

  • eBook ISBN: 978-1-61779-310-3Published: 18 September 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 416

  • Number of Illustrations: 149 b/w illustrations

  • Topics: Neurosciences, Neurochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access