Editors:
- Serves to inspire the next generation of regenerative medicine and tissue engineering technologies
- Features an extensive collection of protocols developed by leading scientists in the field
- Contains practical laboratory guidance to promote successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (34 protocols)
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Front Matter
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Maintenance and Cryopreservation of ES and iPS Cells
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Front Matter
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Maintenance and Cryopreservation of ES and iPS cells
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Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming
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Front Matter
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Generation of Patient-Specific iPS Cells for Clinical Application
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Front Matter
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About this book
Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world. The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies. Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.
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Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.
Editors and Affiliations
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, College of Engineering, University of Arkansas, Fayetteville, USA
Kaiming Ye, Sha Jin
Bibliographic Information
Book Title: Human Embryonic and Induced Pluripotent Stem Cells
Book Subtitle: Lineage-Specific Differentiation Protocols
Editors: Kaiming Ye, Sha Jin
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-1-61779-267-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-266-3Published: 07 September 2011
Softcover ISBN: 978-1-4939-5893-1Published: 23 August 2016
eBook ISBN: 978-1-61779-267-0Published: 08 September 2011
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: XVI, 486
Number of Illustrations: 40 b/w illustrations, 48 illustrations in colour
Topics: Cell Biology, Stem Cells