Overview
- Serves as an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail
- Features cutting-edge, lab ready protocols
- Includes key tips and expert implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 778)
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Table of contents (19 protocols)
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Front Matter
Keywords
About this book
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Editors and Affiliations
Bibliographic Information
Book Title: Single Molecule Enzymology
Book Subtitle: Methods and Protocols
Editors: Gregory I. Mashanov, Christopher Batters
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-261-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-260-1Published: 04 August 2011
Softcover ISBN: 978-1-4939-6190-0Published: 23 August 2016
eBook ISBN: 978-1-61779-261-8Published: 04 August 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 294
Topics: Biochemistry, general, Enzymology