Overview
- Presents well-established and newly emerging techniques as performed by seasoned experts in the field
- Provides easy to follow instruction written in a clear, step-by-step manner
- Includes key tips and implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 777)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.Editors and Affiliations
Bibliographic Information
Book Title: Microtubule Dynamics
Book Subtitle: Methods and Protocols
Editors: Anne Straube
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-252-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-251-9Published: 25 July 2011
Softcover ISBN: 978-1-4939-6185-6Published: 30 April 2017
eBook ISBN: 978-1-61779-252-6Published: 25 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 319
Number of Illustrations: 43 b/w illustrations, 13 illustrations in colour
Topics: Cell Biology, Cell Physiology