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Proprotein Convertases

  • Book
  • © 2011

Overview

Editors:
  1. Majambu Mbikay

    1. Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Nabil G. Seidah

    1. (ICRM), Lab. Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Serves as an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis
  • Provides many cutting-edge lab-ready protocols
  • Includes tips and implementation advice from experts in the field
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 768)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Reminiscences

    1. Front Matter

      Pages 1-1
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    2. On the Discovery of Precursor Processing

      • Donald F. Steiner
      Pages 3-11

    3. The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity

      • Michel Chrétien
      Pages 13-19

  3. Biochemistry and Cell Biology

    1. Front Matter

      Pages 21-21
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    2. The Proprotein Convertases, 20 Years Later

      • Nabil G. Seidah
      Pages 23-57

    3. Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin

      • Ujwal Shinde, Gary Thomas
      Pages 59-106

    4. The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression

      • Lydiane Funkelstein, Vivian Hook
      Pages 107-125

    5. In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies

      • Ajoy Basak, Andrew Chen, Swapan Majumdar, Heather Palmer Smith
      Pages 127-153

    6. Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries

      • Iris Lindberg, Jon R. Appel
      Pages 155-166

    7. Analyses of PCSK9 Post-translational Modifications Using Time-of-Flight Mass Spectrometry

      • Thilina Dewpura, Janice Mayne
      Pages 167-187

  4. Molecular Biology and Genetics

    1. Front Matter

      Pages 189-189
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    2. The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling

      • Philipp Stawowy, Kai Kappert
      Pages 191-206

    3. Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells

      • Nathalie Scamuffa, Peter Metrakos, Fabien Calvo, Abdel-Majid Khatib
      Pages 207-215

    4. Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5′-Untranslated Region

      • Haidy Tadros, Gunther Schmidt, Francine Sirois, Majambu Mbikay
      Pages 217-230

    5. Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease

      • YangXin Fu, Mark W. Nachtigal
      Pages 231-245

    6. Genetic and Functional Characterization of PCSK1

      • Hélène Choquet, Pieter Stijnen, John W.M. Creemers
      Pages 247-253

  5. Experimental Models

    1. Front Matter

      Pages 255-255
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    2. Prohormone Processing in Zebrafish

      • Michael G. Morash, Kelly Soanes, Younes Anini
      Pages 257-271

    3. Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity

      • Babykumari P. Chitramuthu, Hugh P.J. Bennett
      Pages 273-296
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Keywords

About this book

Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions.  In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway.  Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well.

Editors and Affiliations

  • Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Majambu Mbikay

  • (ICRM), Lab. Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Canada

    Nabil G. Seidah

Bibliographic Information

  • Book Title: Proprotein Convertases

  • Editors: Majambu Mbikay, Nabil G. Seidah

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-204-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-203-8Published: 02 August 2011

  • Softcover ISBN: 978-1-4939-6176-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-204-5Published: 02 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 367

  • Topics: Biochemistry, general, Enzymology

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