Overview
- Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells
- Serves a wide variety of scientists, from cell biologists to medical students to pharmacologists
- Features key tips and expert implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 761)
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Table of contents (19 protocols)
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Front Matter
Keywords
About this book
To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.
Editors and Affiliations
Bibliographic Information
Book Title: Cell Cycle Synchronization
Book Subtitle: Methods and Protocols
Editors: Gaspar Banfalvi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-182-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-181-9Published: 12 July 2011
Softcover ISBN: 978-1-4939-6194-8Published: 23 August 2016
eBook ISBN: 978-1-61779-182-6Published: 12 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 295
Topics: Cell Biology, Cell Cycle Analysis