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  • © 2011

Receptor Signal Transduction Protocols

Third Edition

  • Acknowledges the constant evolution of the GPCR field with up-to-date methodologies
  • Contains detailed revisions of chapters from the prior editions as well as a considerable amount of completely new material
  • Features expert tips and key implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 746)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
  2. Developing Models for Studying GPCRs

    1. Front Matter

      Pages 1-1
    2. Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression

      • Richard J. Ward, Elisa Alvarez-Curto, Graeme Milligan
      Pages 21-37
    3. Viral Infection for GPCR Expression in Eukaryotic Cells

      • Antonio Porcellini, Luisa Iacovelli, Antonio De Blasi
      Pages 39-51
    4. Generation of Epitope-Tagged GPCRs

      • Yan Huang, Gary B. Willars
      Pages 53-84
    5. The Use of Site-Directed Mutagenesis to Study GPCRs

      • Alex C. Conner, James Barwell, David R. Poyner, Mark Wheatley
      Pages 85-98
    6. Heterologous Expression of GPCRs in Fission Yeast

      • John Davey, Graham Ladds
      Pages 113-131
  3. Examining GPCR Expression and Agonist-Induced Covalent Modifications

    1. Front Matter

      Pages 133-133
    2. Radioligand Binding Methods for Membrane Preparations and Intact Cells

      • David B. Bylund, Myron L. Toews
      Pages 135-164
    3. Quantification of GPCR mRNA Using Real-Time RT-PCR

      • Trond Brattelid, Finn Olav Levy
      Pages 165-193
    4. Design and Use of Fluorescent Ligands to Study Ligand–Receptor Interactions in Single Living Cells

      • Stephen J. Briddon, Barrie Kellam, Stephen J. Hill
      Pages 211-236
    5. Examining Site-Specific GPCR Phosphorylation

      • Adrian J. Butcher, Andrew B. Tobin, Kok Choi Kong
      Pages 237-249
    6. Ubiquitination of GPCRs

      • Adriana Caballero, Adriano Marchese
      Pages 251-259
  4. Examining Early Events in GPCR Signaling

    1. Front Matter

      Pages 261-261
    2. [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs

      • Rajendra Mistry, Mark R. Dowling, R. A. John Challiss
      Pages 263-275
    3. Using Calcium Imaging as a Readout of GPCR Activation

      • Martin D. Bootman, H. Llewelyn Roderick
      Pages 277-296
    4. Measuring Spatiotemporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors

      • Frank Gesellchen, Alessandra Stangherlin, Nicoletta Surdo, Anna Terrin, Anna Zoccarato, Manuela Zaccolo
      Pages 297-316

About this book

As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues.  Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.

Reviews

From the reviews of the third edition:

“This is an extensive collection of established, new, and updated techniques for the study of the consequences of receptor activation. Each of the 26 chapters offers a specific recipe, with lists of required reagents and equipment, plus notes to aid in use of the protocol. … This book would be most useful to researchers already familiar with fundamental molecular biological or biochemical laboratory techniques.” (Kenneth E. McCarson, Doody’s Book Reviews, February, 2012)

Editors and Affiliations

  • , Dept. Cell Physiology & Pharmacology, University Leicester, Leicester, United Kingdom

    Gary B. Willars, R.A. John Challiss

Bibliographic Information

  • Book Title: Receptor Signal Transduction Protocols

  • Book Subtitle: Third Edition

  • Editors: Gary B. Willars, R.A. John Challiss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-126-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-125-3Published: 27 June 2011

  • Softcover ISBN: 978-1-4939-5625-8Published: 23 August 2016

  • eBook ISBN: 978-1-61779-126-0Published: 24 May 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 479

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access