Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 744)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
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In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.Editors and Affiliations
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Graduate School of Horticulture, Chiba University, Chiba, Japan
Hiroaki Kodama
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Evolutionary Biology, Research Institute of, Tokyo, Setagaya, Japan
Atsushi Komamine
Bibliographic Information
Book Title: RNAi and Plant Gene Function Analysis
Book Subtitle: Methods and Protocols
Editors: Hiroaki Kodama, Atsushi Komamine
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-123-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-122-2Published: 04 May 2011
Softcover ISBN: 978-1-62703-932-1Published: 29 November 2014
eBook ISBN: 978-1-61779-123-9Published: 30 April 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 244
Topics: Plant Genetics and Genomics