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Cystic Fibrosis

Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis

  • Book
  • © 2011

Overview

Editors:
  1. Margarida D. Amaral

    1. Faculty of Sciences, Centre for Biodiversity & Functional, University of Lisboa, Lisboa, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Karl Kunzelmann

    1. , Department of Physiology, University of Regensburg, Regensburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Features cutting-edge protocols from the highly supportive CF scientific community
  • Contains expert tips and key implementation advice
  • Focuses on cystic fibrosis pathophysiology and Omics studies with an emphasis on practical approaches
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 742)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Pathophysiology of Cystic Fibrosis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

  3. Pathophysiology Of Cystic Fibrosis

    1. Introduction to Section I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology

      • Mark T. Clunes, Richard C. Boucher
      Pages 3-14

    2. Imaging CFTR Protein Localization in Cultured Cells and Tissues

      • Silvia M. Kreda, Martina Gentzsch
      Pages 15-33

    3. CFTR Regulation of Epithelial Sodium Channel

      • Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, Bakhrom K. Berdiev
      Pages 35-50

    4. Methods for Evaluating Inflammation in Cystic Fibrosis

      • Assem G. Ziady, Pamela B. Davis
      Pages 51-76

    5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures

      • Erin N. Worthington, Robert Tarran
      Pages 77-92

    6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands

      • Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyung-Ju Cho, Mauri E. Krouse, Jin V. Wu et al.
      Pages 93-112

    7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia

      • Carla M.P. Ribeiro
      Pages 113-126

    8. Identification and Quantification of Mucin Expression

      • Kristina A. Thomsson, Gunnar C. Hansson
      Pages 127-141

    9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis

      • Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard, Niels Høiby
      Pages 143-171

    10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

      • Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, Marc Chanson
      Pages 173-185

  4. Omic Approaches to Study Cystic Fibrosis

    1. Front Matter

      Pages 187-187
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  5. Omic Approaches To Study Cystic Fibrosis

    1. Introduction to Section II: Omics in the Biology of Cystic Fibrosis

      • William E. Balch
      Pages 189-191

    2. Microarray mRNA Expression Profiling to Study Cystic Fibrosis

      • Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, Paul B. McCray Jr
      Pages 193-212

    3. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)

      • Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, Aleksander Edelman
      Pages 213-225

    4. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis

      • William E. Balch, John R. Yates III
      Pages 227-247

    5. Functional Genomics Assays to Study CFTR Traffic and ENaC Function

      • Joana Almaça, Shehrazade Dahimène, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok et al.
      Pages 249-264

    6. New Lipidomic Approaches in Cystic Fibrosis

      • Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, Aleksander Edelman
      Pages 265-278

  6. Resources

    1. Front Matter

      Pages 279-279
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Keywords

About this book

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.  Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis.  Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.

Editors and Affiliations

  • Faculty of Sciences, Centre for Biodiversity & Functional, University of Lisboa, Lisboa, Portugal

    Margarida D. Amaral

  • , Department of Physiology, University of Regensburg, Regensburg, Germany

    Karl Kunzelmann

Bibliographic Information

  • Book Title: Cystic Fibrosis

  • Book Subtitle: Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis

  • Editors: Margarida D. Amaral, Karl Kunzelmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-120-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-119-2Published: 11 May 2011

  • Softcover ISBN: 978-1-4939-6282-2Published: 23 August 2016

  • eBook ISBN: 978-1-61779-120-8Published: 06 May 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 384

  • Topics: Molecular Medicine, Human Genetics, Laboratory Medicine

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