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Mammalian Cell Viability

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Martin J. Stoddart

    1. AO Research Institute, Davos Platz, Switzerland

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  • Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment
  • Facilitates the multiplexing of methods for the greatest levels of success
  • Contains expert tips and key implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 740)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
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  2. Cell Viability Assays: Introduction

    • Martin J. Stoddart
    Pages 1-6

  3. Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

    • Kristine S. Louis, Andre C. Siegel
    Pages 7-12

  4. Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay

    • László Kupcsik
    Pages 13-19

  5. WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

    • Martin J. Stoddart
    Pages 21-25

  6. Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

    • Ewa M. Czekanska
    Pages 27-32

  7. The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability

    • Ning Ke, Xiaobo Wang, Xiao Xu, Yama A. Abassi
    Pages 33-43

  8. Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic Assays

    • Caroline Woolston, Stewart Martin
    Pages 45-56

  9. Annexin V/7-AAD Staining in Keratinocytes

    • Maya Zimmermann, Norbert Meyer
    Pages 57-63

  10. Measurement of Caspase Activity: From Cell Populations to Individual Cells

    • Gabriela Paroni, Claudio Brancolini
    Pages 65-79

  11. Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

    • Donald Wlodkowic, Joanna Skommer, Zbigniew Darzynkiewicz
    Pages 81-89

  12. Multiplexing Cell Viability Assays

    • Helga H. J. Gerets, Stéphane Dhalluin, Franck A. Atienzar
    Pages 91-101

  13. Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death

    • Terry L. Riss, Richard A. Moravec, Andrew L. Niles
    Pages 103-114

  14. Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast–Collagen–GAG Constructs

    • Heather M. Powell, Alexis D. Armour, Steven T. Boyce
    Pages 115-126

  15. Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

    • Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, Sibylle Grad
    Pages 127-140

  16. Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections

    • Katharina Jähn, Martin J. Stoddart
    Pages 141-148

  17. Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay

    • Won-Kyu Ju, Keun-Young Kim
    Pages 149-156

  18. Exploiting the Liberation of Zn2+ to Measure Cell Viability

    • Christian J. Stork, Yang V. Li
    Pages 157-163

  19. Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

    • Karim Oudina, Adeline Cambon-Binder, Delphine Logeart-Avramoglou
    Pages 165-178

  20. Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test

    • Sophie Verrier, Alina Zoladek, Ioan Notingher
    Pages 179-189
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Keywords

About this book

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Editors and Affiliations

  • AO Research Institute, Davos Platz, Switzerland

    Martin J. Stoddart

Bibliographic Information

  • Book Title: Mammalian Cell Viability

  • Book Subtitle: Methods and Protocols

  • Editors: Martin J. Stoddart

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-108-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-107-9Published: 12 April 2011

  • Softcover ISBN: 978-1-4939-6153-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-108-6Published: 06 April 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 240

  • Topics: Cell Biology, Cell Culture

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