Overview
- Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment
- Facilitates the multiplexing of methods for the greatest levels of success
- Contains expert tips and key implementation advice
Part of the book series: Methods in Molecular Biology (MIMB, volume 740)
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Table of contents (21 protocols)
Keywords
About this book
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
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Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.Editors and Affiliations
Bibliographic Information
Book Title: Mammalian Cell Viability
Book Subtitle: Methods and Protocols
Editors: Martin J. Stoddart
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-108-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-107-9Published: 12 April 2011
Softcover ISBN: 978-1-4939-6153-5Published: 23 August 2016
eBook ISBN: 978-1-61779-108-6Published: 06 April 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 240
Topics: Cell Biology, Cell Culture