Editors:
- Provides a precious resource for scientists interested in new ideas in gene technology
- Features practical protocols from the cutting-edge of this fast-paced field
- Includes expert tips and key implementation advice crucial for getting optimal results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 738)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
About this book
The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.Editors and Affiliations
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Biological Research Center, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary
Gyula Hadlaczky
Bibliographic Information
Book Title: Mammalian Chromosome Engineering
Book Subtitle: Methods and Protocols
Editors: Gyula Hadlaczky
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-099-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-098-0Published: 06 April 2011
Softcover ISBN: 978-1-4939-5775-0Published: 23 August 2016
eBook ISBN: 978-1-61779-099-7Published: 23 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: IX, 257
Topics: Human Genetics, Genetic Engineering