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Cancer Cytogenetics

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Lynda J. Campbell

    1. Victorian Cancer Cytogenetics Service, St. Vincent's Hospital Melbourne, Fitzroy, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 730)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction

    • Lynda J. Campbell
    Pages 1-2

  3. Evolution of Cytogenetic Methods in the Study of Cancer

    • Lynda J. Campbell
    Pages 3-12

  4. Fluorescence In Situ Hybridization Methods and Troubleshooting Applied to Fixed Cell Suspensions

    • John Swansbury, Toon Min, Shilani Aruliah
    Pages 13-31

  5. Chronic Myeloid Leukemia: Cytogenetic Methods and Applications for Diagnosis and Treatment

    • Christine M. Morris
    Pages 33-61

  6. Cytogenetic Analysis in Acute Myeloid Leukaemia

    • Lynda J. Campbell, Joanne S. White
    Pages 63-77

  7. Cytogenetics in Myelodysplastic Syndromes

    • Kazuma Ohyashiki, Atsushi Kodama, Junko H. Ohyashiki
    Pages 79-88

  8. Cytogenetics of Myeloproliferative Neoplasms

    • Lynda J. Campbell
    Pages 89-98

  9. Acute Lymphoblastic Leukaemia

    • Claire Schwab, Christine J. Harrison
    Pages 99-117

  10. Cytogenetic Methods in Chronic Lymphocytic Leukemia

    • Claudia Haferlach, Ulrike Bacher
    Pages 119-130

  11. Genetic Abnormalities in Non-Hodgkin’s Lymphoma as Revealed by Conventional and Molecular Cytogenetics Methods of Analyses

    • Suresh C. Jhanwar, Ryan C. Denley
    Pages 131-148

  12. Metaphase Cytogenetic Techniques in Multiple Myeloma

    • Jeffrey R. Sawyer
    Pages 149-158

  13. Detection of Chromosome Abnormalities Using Cytoplasmic Immunoglobulin Staining and FISH in Myeloma

    • Bruce R. Mercer, Kathleen C. Rayeroux
    Pages 159-171

  14. Cytogenetics of Solid Tumours

    • Robyn Lukeis, Mary Suter
    Pages 173-187

  15. Fluorescence In Situ Hybridization on Formalin-Fixed, Paraffin-Embedded Tissue Sections

    • Adrian Zordan
    Pages 189-202

  16. The Use of M-FISH and M-BAND to Define Chromosome Abnormalities

    • Ruth N. MacKinnon, Ilse Chudoba
    Pages 203-218

  17. Utility of Array Comparative Genomic Hybridization in Cytogenetic Analysis

    • Rashmi R. Singh, K-John J. Cheung, Douglas E. Horsman
    Pages 219-234

  18. Single Nucleotide Polymorphism Microarray Analysis of Genetic Alterations in Cancer

    • Charles G. Mullighan
    Pages 235-258

  19. Cytogenetic Analysis and Reporting

    • Lynda J. Campbell
    Pages 259-268

  20. Back Matter

    Pages 269-273
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Keywords

About this book

Cytogenetic studies of malignancy have become an essential tool in the clinical management of cancer patients. Cancer Cytogenetics: Methods and Protocols presents eminently practical key cytogenetic and FISH techniques for every stage of diagnostic service. Experts in the field describe detailed cytogenetic analysis methods, fluorescence in situ hybridization and array methods currently being applied to investigate and diagnose different varieties of cancer. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols. The authors of the various chapters have also provided extensive notes to guide individuals who are new to these methods through the pitfalls that bedevil all such testing.

Authoritative and accessible, Cancer Cytogenetics: Methods and Protocols serves as an ideal guide to scientists of all backgrounds, allowing them to either establish new techniques in their laboratories or find the different variations of standard methods helpful in improving their results.

Editors and Affiliations

  • Victorian Cancer Cytogenetics Service, St. Vincent's Hospital Melbourne, Fitzroy, Australia

    Lynda J. Campbell

Bibliographic Information

  • Book Title: Cancer Cytogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Lynda J. Campbell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-074-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-073-7Published: 16 March 2011

  • Softcover ISBN: 978-1-4939-5655-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-074-4Published: 23 March 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 273

  • Topics: Cancer Research, Cytogenetics

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