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  • © 2011

cDNA Libraries

Methods and Applications

  • Captures the current state of this ever-developing and powerful technology
  • Features practical methodologies, ready for the lab
  • Contains vital implementation advice from experts in the field
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 729)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Enhanced Approaches to Inherent Problems

    1. Front Matter

      Pages 1-1
    2. Construction of Improved Yeast Two-Hybrid Libraries

      • Richard H. Maier, Christina J. Maier, Kamil Önder
      Pages 71-84
    3. Normalization of Full-Length-Enriched cDNA

      • Ekaterina A. Bogdanova, Ekaterina V. Barsova, Irina A. Shagina, Alexander Scheglov, Veronika Anisimova, Laura L. Vagner et al.
      Pages 85-98

About this book

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.  In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries.  The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.

Editors and Affiliations

  • , Dept. Plant Sciences & Plant Pathology, Montana State University, Bozeman, USA

    Chaofu Lu

  • Institute of Biological Chemistry, Washington State University, Pullman, USA

    John Browse, James G. Wallis

Bibliographic Information

  • Book Title: cDNA Libraries

  • Book Subtitle: Methods and Applications

  • Editors: Chaofu Lu, John Browse, James G. Wallis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-065-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-064-5Published: 03 March 2011

  • Softcover ISBN: 978-1-4939-5796-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-065-2Published: 02 March 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 275

  • Topics: Human Genetics, Gene Expression

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access