Editors:
- Serves as a toolbox of methodologies currently used in the field of protein microarray study
- Features step-by-step protocols ready for the lab
- Contains expert tips and vital implementation advice to ensure success
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 723)
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Table of contents (22 protocols)
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Front Matter
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Protein-Detecting Analytical Microarrays
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Front Matter
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Antigen Microarrays for Immunoprofiling
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Front Matter
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Protein Function Microarrays
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Front Matter
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Strategies for Validation of Candidate Targets
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Front Matter
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About this book
Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.
Reviews
From the reviews:
“This is a well-illustrated book with concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to detect disease. … The book is intended for scientists, but it also will be of immense help to graduate students and researchers interested protein microarrays. … a well-done resource on the methodologies currently used in protein microarrays to promote a cell-level biochemical understanding of human disease processes that could revolutionize drug development.” (Omer Iqbal, Doody’s Review Services, July, 2011)Editors and Affiliations
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Cancer Vaccine Center, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, USA
Catherine J. Wu
Bibliographic Information
Book Title: Protein Microarray for Disease Analysis
Book Subtitle: Methods and Protocols
Editors: Catherine J. Wu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-043-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-042-3Published: 03 March 2011
Softcover ISBN: 978-1-4939-6174-0Published: 23 August 2016
eBook ISBN: 978-1-61779-043-0Published: 03 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 373
Topics: Protein Science, Microarrays