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  • © 2011

The Plant Cell Wall

Methods and Protocols

Editors:

  • Features many recently developed methods, becoming more widely used with the development of advanced instrumentation and technology
  • Contains detailed protocols to study plant cell wall structure, biochemical composition, and metabolism, to name a few areas
  • Includes expert tips and vital implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 715)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
  2. Plant Tissue Cultures

    • Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, Hiroo Fukuda
    Pages 1-20
  3. Computerized Molecular Modeling of Carbohydrates

    • Alfred D. French, Glenn P. Johnson
    Pages 21-42
  4. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

    • Markus Günl, Florian Kraemer, Markus Pauly
    Pages 43-54
  5. Carbohydrate Gel Electrophoresis

    • Florence Goubet, Paul Dupree, Katja Salomon Johansen
    Pages 81-92
  6. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

    • Iben Sørensen, William G. T. Willats
    Pages 115-121
  7. Analysing Cellulose Biosynthesis with Confocal Microscopy

    • Meera Nair, Seth DeBolt
    Pages 141-152
  8. Visual Mapping of Cell Wall Biosynthesis

    • Yumiko Sakuragi, Morten H. H. Nørholm, Henrik V. Scheller
    Pages 153-167
  9. Atomic Force Microscopy of Plant Cell Walls

    • Andrew R. Kirby
    Pages 169-178
  10. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls

    • Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, Roger H. Newman
    Pages 179-196
  11. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

    • Derek T. A. Lamport, Li Tan, Marcia J. Kieliszewski
    Pages 209-219
  12. New Insights into the Control of Cell Growth

    • Claudia Blaukopf, Matthäus Z. Krol, Georg J. Seifert
    Pages 221-244
  13. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

    • Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek Jr., Chessa A. Goss, Virginia Lai
    Pages 273-290

About this book

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.  The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

Editors and Affiliations

  • School of Natural Sciences, Dept. of Botany & Plant Science, National University of Ireland, Galway, Galway, Ireland

    Zoë A. Popper

Bibliographic Information

  • Book Title: The Plant Cell Wall

  • Book Subtitle: Methods and Protocols

  • Editors: Zoë A. Popper

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-008-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-007-2Published: 12 January 2011

  • Softcover ISBN: 978-1-4939-6179-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-008-9Published: 11 January 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 310

  • Topics: Plant Sciences, Plant Physiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access