Overview
- Editors:
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Trevor A. Thorpe
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Dept. Biological Sciences, University of Calgary, Calgary, Canada
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Edward C. Yeung
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, Department of Biological Sciences, University of Calgary, Calgary, Canada
- Presents detailed zygotic embryo isolation techniques with a wide variety of applications
- Serves as a quick, convenient reference source
- Includes tips from the labs of experts, well-versed in the methods and protocols provided
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Table of contents (24 protocols)
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Culture of Zygotic Embryos: Fundamental Studies
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- Tegan M. Haslam, Edward C. Yeung
Pages 3-15
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- Nicole S. Ramesar-Fortner, Edward C. Yeung
Pages 29-37
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- Florent Engelmann, Bernard Malaurie, Oulo N’Nan
Pages 63-72
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Application of Embryo Culture Techniques: Embryo Rescue
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- Xiuli Shen, Fred G. Gmitter Jr., Jude W. Grosser
Pages 75-92
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- Nalini Mallikarjuna, Fred J. Muehlbauer
Pages 93-105
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- Hector Gordon Nuñez-Palenius, Rafael RamÃrez-Malagón, Neftalà Ochoa-Alejo
Pages 107-115
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- Pascal Geerts, André Toussaint, Guy Mergeai, Jean-Pierre Baudoin
Pages 117-129
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- Richard Fratini, Maria L. Ruiz
Pages 131-139
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- Ajoy Kumar Roy, Devendra Ram Malaviya, Pankaj Kaushal
Pages 141-151
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Cryopreservation of Embryos
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Front Matter
Pages 153-153
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- Anna De Carlo, Maurizio Lambardi, Elif Aylin Ozudogru
Pages 185-200
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- Ana M. Vieitez, M. Carmen San-José, Elena Corredoira
Pages 201-213
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- Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, Hebe Rey
Pages 215-225
About this book
A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.
Editors and Affiliations
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Dept. Biological Sciences, University of Calgary, Calgary, Canada
Trevor A. Thorpe
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, Department of Biological Sciences, University of Calgary, Calgary, Canada
Edward C. Yeung