Plant Embryo Culture

1. Front Matter

   Pages i-xi

   PDF

2. Culture of Zygotic Embryos: Fundamental Studies

   1. Front Matter

      Pages 1-1

      PDF

   2. Zygotic Embryo Culture: An Overview

      • Tegan M. Haslam, Edward C. Yeung

      Pages 3-15

   3. In Vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture

      • Takashi Okamoto

      Pages 17-27

   4. Canola Zygotic Embryo Culture

      • Nicole S. Ramesar-Fortner, Edward C. Yeung

      Pages 29-37

   5. Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro

      • Sergio J. Ochatt

      Pages 39-52

   6. In Vitro Culture and Germination of Terrestrial Asian Orchid Seeds

      • Yung-I. Lee

      Pages 53-62

   7. In Vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos

      • Florent Engelmann, Bernard Malaurie, Oulo N’Nan

      Pages 63-72

3. Application of Embryo Culture Techniques: Embryo Rescue

   1. Front Matter

      Pages 73-73

      PDF

   2. Immature Embryo Rescue and Culture

      • Xiuli Shen, Fred G. Gmitter Jr., Jude W. Grosser

      Pages 75-92

   3. Chickpea Hybridization Using In Vitro Techniques

      • Nalini Mallikarjuna, Fred J. Muehlbauer

      Pages 93-105

   4. Muskmelon Embryo Rescue Techniques Using In Vitro Embryo Culture

      • Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, Neftalí Ochoa-Alejo

      Pages 107-115

   5. Phaseolus Immature Embryo Rescue Technology

      • Pascal Geerts, André Toussaint, Guy Mergeai, Jean-Pierre Baudoin

      Pages 117-129

   6. Wide Crossing in Lentil through Embryo Rescue

      • Richard Fratini, Maria L. Ruiz

      Pages 131-139

   7. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques

      • Ajoy Kumar Roy, Devendra Ram Malaviya, Pankaj Kaushal

      Pages 141-151

4. Cryopreservation of Embryos

   1. Front Matter

      Pages 153-153

      PDF

   2. Cryopreservation of Embryos: An Overview

      • Florent Engelmann

      Pages 155-184

   3. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm

      • Anna De Carlo, Maurizio Lambardi, Elif Aylin Ozudogru

      Pages 185-200

   4. Cryopreservation of Zygotic Embryonic Axes and Somatic Embryos of European Chestnut

      • Ana M. Vieitez, M. Carmen San-José, Elena Corredoira

      Pages 201-213

   5. Cryopreservation of Ilex Immature Zygotic Embryos

      • Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, Hebe Rey

      Pages 215-225

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

• Cryopreservation
• Embryo development
• Embryo rescue
• Explants
• In vitro culture
• Isolation
• Organogenesis
• Somatic embryogenesis
• Transformation protocols
• Zygotic embryos
• Cell culture

• Dept. Biological Sciences, University of Calgary, Calgary, Canada

  Trevor A. Thorpe

• , Department of Biological Sciences, University of Calgary, Calgary, Canada

  Edward C. Yeung

Policies and ethics