Morphology Methods

1. Front Matter

   Pages i-xvi

   PDF

2. Introduction to Molecular Methods

   • Ricardo V. Lloyd

   Pages 1-10

3. Laser Capture Microdissection

   • Ricardo V. Lloyd

   Pages 11-25

4. In Situ Hybridization

   • Long Jin, Xiang Qian, Ricardo V. Lloyd

   Pages 27-46

5. In Situ Detection of Infectious Agents

   • Gary W. Procop, Randall Hayden

   Pages 47-65

6. Fluorescent In Situ Hybridization

   • Susan Sheldon

   Pages 67-89

7. Practical Applications of the FISH Technique

   • George Kontogeorgos, Nikiforos Kapranos, Eleni Thodou

   Pages 91-111

8. Tyramide Amplification Methods for In Situ Hybridization

   • John L. Frater, Raymond R. Tubbs

   Pages 113-128

9. Ultrastructural In Situ Hybridization

   • Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe

   Pages 129-144

10. Quantitation of In Situ Hybridization Analysis

    • Lars-Inge Larsson

    Pages 145-163

11. Confocal Laser Scanning Microscopy

    • Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe

    Pages 165-180

12. Polymerase Chain Reaction

    • Yuri E. Nikiforov, Philip N. Howles

    Pages 181-207

13. Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction

    • Omar Bagasra, Lisa E. Bobroski, Muhammad Amjad

    Pages 209-227

14. Immunohistochemistry: Theory and Practice

    • Patrick C. Roche, Eric D. Hsi

    Pages 229-237

15. Antigen Retrieval Technique for Immunohistochemistry

    • Clive R. Taylor, Shan-Rong Shi

    Pages 239-265

16. Tyramide Amplification in Immunohistochemistry

    • Naoko Sanno, Akira Teramoto, R. Yoshiyuki Osamura

    Pages 267-276

17. Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions

    • Paul J. Kurtin

    Pages 277-322

18. Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors

    • Mark R. Wick, Lisa A. Cerilli

    Pages 323-360

19. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions

    • Ricardo V. Lloyd

    Pages 361-374

20. Ultrastructural Immunohistochemistry

    • Sergio Vidal, Eva Horvath, Kalman Kovacs

    Pages 375-402

The past several decades have witnessed an impressive array of conceptual and techno­ logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula­ tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab­ th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under­ standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

• DNA
• FISH
• PCR
• hybridization
• molecular biology
• tissue

"The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. " -Doody's Health Science Book Review Journal

"This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the "FISH" technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques." - Journal of Pediatric Endocrinology and Metabolism

• Mayo Clinic, Rochester, USA

  Ricardo V. Lloyd

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