Skip to main content
SpringerLink
Log in
Book cover

Quorum Sensing

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Kendra P. Rumbaugh

    1. Health Sciences Center, Department of Surgery, Texas Tech University, Lubbock, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Features in-depth protocols on detecting, isolating, characterizing QS signals, function determination, and QS disruption strategies
  • Contributions from expert researchers who have experienced the field from its beginnings to the current cutting-edge studies
  • Includes key notes on avoiding known protocol pitfalls and troubleshooting potential problems

Part of the book series: Methods in Molecular Biology (MIMB, volume 692)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Detection, Isolation, and Characterization of Quorum Sensing Compounds

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum

      • Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, Robert J. C. McLean
      Pages 3-19

    3. Detection of 2-Alkyl-4-Quinolones Using Biosensors

      • Stephen P. Diggle, Matthew P. Fletcher, Miguel Cámara, Paul Williams
      Pages 21-30

    4. FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds

      • Sathish Rajamani, Richard Sayre
      Pages 31-46

    5. Isolation of agr Quorum Sensing Autoinducers

      • Naomi Balaban
      Pages 47-59

    6. Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines

      • François Lépine, Eric Déziel
      Pages 61-69

    7. Detection of Autoinducer (AI-2)-Like Activity in Food Samples

      • Kirthiram K. Sivakumar, Palmy R. Jesudhasan, Suresh D. Pillai
      Pages 71-82

    8. Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments

      • Peter Edmonson, Desmond Stubbs, William Hunt
      Pages 83-100

    9. Determination of Acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples

      • Colin A. Lowery, Gunnar F. Kaufmann, Kim D. Janda
      Pages 101-111

    10. Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing

      • Ali Alagely, Sathish Rajamani, Max Teplitski
      Pages 113-130

  3. Determining the Function of Autoinducers In vivo

    1. Front Matter

      Pages 131-131
      Download chapter PDF

    2. Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules

      • Vladimir V. Kravchenko, Richard J. Ulevitch, Gunnar F. Kaufmann
      Pages 133-145

    3. Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

      • Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
      Pages 147-157

    4. Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers

      • Mair E. A. Churchill, Hiruy M. Sibhatu, Charis L. Uhlson
      Pages 159-171

    5. Global Expression Analysis of Quorum-Sensing Controlled Genes

      • Martin Schuster
      Pages 173-187

    6. Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System

      • Brian K. Hammer, Sine Lo Svenningsen
      Pages 189-206

    7. Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids

      • Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, Marvin Whiteley
      Pages 207-217

    8. Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans

      • Aurélie Deveau, Deborah A. Hogan
      Pages 219-233

    9. Design of Synthetic Mammalian Quorum-Sensing Systems

      • Wilfried Weber, Martin Fussenegger
      Pages 235-249
Back to top

Keywords

About this book

Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls. Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.

Editors and Affiliations

  • Health Sciences Center, Department of Surgery, Texas Tech University, Lubbock, USA

    Kendra P. Rumbaugh

Bibliographic Information

  • Book Title: Quorum Sensing

  • Book Subtitle: Methods and Protocols

  • Editors: Kendra P. Rumbaugh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-971-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60761-970-3Published: 05 November 2010

  • Softcover ISBN: 978-1-4939-6162-7Published: 23 August 2016

  • eBook ISBN: 978-1-60761-971-0Published: 29 October 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 315

  • Topics: Microbiology, Bacteriology, Laboratory Medicine

Publish with us

Policies and ethics

Back to top

Search

Navigation