Overview
- Editors:
-
-
Bimal D.M. Theophilus
-
NHS Foundation Trust, Department of Haematology, Birmingham Childrens Hospital, Birmingham, United Kingdom
-
Ralph Rapley
-
Dept. Biosciences, University of Hertfordshire, Hatfield, Herts., United Kingdom
- Fully updates the popular previous edition
- Offers cutting-edge techniques, such as those for microarrays, with the underlying basis of the method for successful implementation
- Features key tips from the expert contributors drawing upon their extensive experience
- Includes supplementary material: sn.pub/extras
Access this book
Other ways to access
Table of contents (20 protocols)
-
-
-
-
- Mike Mitchell, Jacqueline Cutler
Pages 17-33
-
-
- Kenneth E. Pierce, Lawrence J. Wangh
Pages 47-66
-
-
- Duncan Ayers, Mark Platt, Farzad Javad, Philip J. R. Day
Pages 75-95
-
- Petra G. C. Eijk - Van Os, Jan P. Schouten
Pages 97-126
-
- Claude Houdayer, Catherine Dehainault, Marion Gauthier-Villars, Dominique Stoppa-Lyonnet
Pages 127-142
-
- Jayne A. L. Minton, Sarah E. Flanagan, Sian Ellard
Pages 143-153
-
- Cathy Meaney, Gail Norbury
Pages 155-172
-
- Yvonne Wallis, Natalie Morrell
Pages 173-185
-
- Alexander Loy, Michael Pester, Doris Steger
Pages 187-206
-
- Kathy Mann, Erwin Petek, Barbara Pertl
Pages 207-226
-
-
-
- Ann Williams, D. Chas Mangham
Pages 257-268
-
- Joanne Mason, Mike Griffiths
Pages 269-280
-
- Gillian Mellars, Keith Gomez
Pages 281-291
About this book
Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method.
Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.
Editors and Affiliations
-
NHS Foundation Trust, Department of Haematology, Birmingham Childrens Hospital, Birmingham, United Kingdom
Bimal D.M. Theophilus
-
Dept. Biosciences, University of Hertfordshire, Hatfield, Herts., United Kingdom
Ralph Rapley