Overview
- Editors:
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Sukriti Nag
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Banting Institute, Dept. Laboratory Med. & Pathobiology, University of Toronto, Toronto, Canada
- Reviews the relevant aspects of brain biology then delves into in-depth, practical laboratory protocols Explores beyond the blood-brain barrier into other vital neural barriers Features tips for completing the protocols successfully, relayed by the expert contributors from their wealth of experience
- Includes supplementary material: sn.pub/extras
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Table of contents (25 protocols)
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Biology of the Barriers
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- Paula Dore-Duffy, Kristen Cleary
Pages 49-68
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- Conrad E. Johanson, Edward G. Stopa, Paul N. McMillan
Pages 101-131
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- E. Aaron Runkle, David A. Antonetti
Pages 133-148
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- Ananda Weerasuriya, Andrew P. Mizisin
Pages 149-173
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Imaging the Barriers
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Front Matter
Pages 175-175
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- Janet L. Manias, Anish Kapadia, Sukriti Nag
Pages 177-192
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- Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, Joseph D. Fenstermacher
Pages 193-212
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- Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, Robin P. Choudhury
Pages 213-227
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- Andrea Kassner, Rebecca Thornhill
Pages 229-245
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- Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, Katarzyna M. Dziegielewska
Pages 247-265
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Molecular Techniques to Study the Blood–Brain Barrier
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Front Matter
Pages 273-273
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Molecular Techniques to Study the Blood-Brain Barrier
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- Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, Alexandre Prat
Pages 275-295
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- Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, Joel S. Pachter
Pages 297-311
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- Gary N. Y. Chan, Reina Bendayan
Pages 313-336
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- Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, Danica B. Stanimirovic
Pages 337-353
About this book
Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cutting-edge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, The Blood-Brain and Other Neural Barriers: Reviews and Protocols will aid the research efforts of not only graduate students but also more experienced investigators and support future studies of these vital systems.
Editors and Affiliations
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Banting Institute, Dept. Laboratory Med. & Pathobiology, University of Toronto, Toronto, Canada
Sukriti Nag
