Enzyme Stabilization and Immobilization

1. Front Matter

   Pages i-x

   PDF

2. Introduction to the Field of Enzyme Immobilization and Stabilization

   • Michael J. Moehlenbrock, Shelley D. Minteer

   Pages 1-7

3. Stabilization of Enzymes Through Encapsulation in Liposomes

   • Makoto Yoshimoto

   Pages 9-18

4. Micellar Enzymology for Thermal, pH, and Solvent Stability

   • Shelley D. Minteer

   Pages 19-24

5. Lipase Activation and Stabilization in Room-Temperature Ionic Liquids

   • Joel L. Kaar

   Pages 25-35

6. Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems

   • Andreas Buthe, Songtao Wu, Ping Wang

   Pages 37-48

7. Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

   • Allan E. David, Arthur J. Yang, Nam Sun Wang

   Pages 49-66

8. Nanoporous Gold for Enzyme Immobilization

   • Keith J. Stine, Kenise Jefferson, Olga V. Shulga

   Pages 67-83

9. Enzyme Stabilization via Bio-templated Silicification Reactions

   • Glenn R. Johnson, Heather R. Luckarift

   Pages 85-97

10. The Immobilization of Enzyme on Eupergit® Supports by Covalent Attachment

    • Zorica D. Knežević-Jugović, Dejan I. Bezbradica, Dušan Ž. Mijin, Mirjana G. Antov

    Pages 99-111

11. Micellar Polymer Encapsulation of Enzymes

    • Sabina Besic, Shelley D. Minteer

    Pages 113-131

12. Enzyme Stabilization via Cross-Linked Enzyme Aggregates

    • Munishwar N. Gupta, Smita Raghava

    Pages 133-145

13. Enzyme Immobilization in Polyelectrolyte Microcapsules

    • Michael J. McShane

    Pages 147-154

14. Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

    • Nenad B. Milosavić, Radivoje M. Prodanović

    Pages 155-163

15. Enzyme–Nanoparticle Conjugates for Biomedical Applications

    • Alexey A. Vertegel, Vladimir Reukov, Victor Maximov

    Pages 165-182

16. Enzyme Nanoparticle Fabrication: Magnetic Nanoparticle Synthesis and Enzyme Immobilization

    • Patrick A. Johnson, Hee Joon Park, Ashley J. Driscoll

    Pages 183-191

17. Cytochrome c Stabilization and Immobilization in Aerogels

    • Amanda S. Harper-Leatherman, Jean Marie Wallace, Debra R. Rolison

    Pages 193-205

18. Kinetic Measurements for Enzyme Immobilization

    • Michael J. Cooney

    Pages 207-225

19. Back Matter

    Pages 227-229

    PDF

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

• Enzyme encapsulation
• Enzyme immobilization
• Gel entrapment
• Thermal stability
• pH tolerance

• Department of Chemistry, St. Louis University, St. Louis, USA

  Shelley D. Minteer

Policies and ethics