Oral Biology

1. Front Matter

   Pages i-xiv

   PDF

2. Saliva Studies

   1. Front Matter

      Pages 1-1

      PDF

   2. Gene Therapy of Salivary Diseases

      • Bruce J. Baum, Janik Adriaansen, Ana P. Cotrim, Corinne M. Goldsmith, Paola Perez, Senrong Qi et al.

      Pages 3-20

   3. Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications

      • Bradley Stephen Henson, David T. Wong

      Pages 21-30

   4. Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting

      • Shen Hu, Jiang Jiang, David T. Wong

      Pages 31-41

   5. Transcriptomic Analyses of Saliva

      • Viswanathan Palanisamy, David T. Wong

      Pages 43-51

3. Oral Microbiology

   1. Front Matter

      Pages 53-53

      PDF

   2. The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques

      • José F. Siqueira Jr, Isabela N. Rôças

      Pages 55-69

   3. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

      • José F. Siqueira Jr, Mitsuo Sakamoto, Alexandre S. Rosado

      Pages 71-85

   4. Protocols to Study the Physiology of Oral Biofilms

      • José A. Lemos, Jacqueline Abranches, Hyun Koo, Robert E. Marquis, Robert A. Burne

      Pages 87-102

   5. Adhesion of Yeast and Bacteria to Oral Surfaces

      • Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Ann R. Holmes

      Pages 103-124

   6. Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction

      • Stephen M. Hamlet

      Pages 125-140

   7. Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy

      • Abdul Aziz, Dikesh Parmar, Andrew McNaughton, Geoffrey R. Tompkins

      Pages 141-150

   8. Characterization of Anti-competitor Activities Produced by Oral Bacteria

      • Fengxia Qi, Jens Kreth

      Pages 151-166

   9. Natural Transformation of Oral Streptococci

      • Fernanda Cristina Petersen, Anne Aamdal Scheie

      Pages 167-180

   10. Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis

       • Shannon M. Wallet, Jin Chung, Martin Handfield

       Pages 181-195

   11. Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System

       • Nicholas C.K. Heng, Jo-Ann L. Stanton

       Pages 197-218

   12. Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps

       • Erwin Lamping, Richard D. Cannon

       Pages 219-250

4. Cells and Tissues

   1. Front Matter

      Pages 251-251

      PDF

   2. Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression

      • Katja Närhi, Irma Thesleff

      Pages 253-267

It is generally recognized that the knowledge and research base that underpins dentistry lies in the biological and physical sciences. In this context, the major advances in these sciences over the past two decades have come through the application of molecular bi- ogy and nanotechnology. These advances are currently impacting on the diagnosis and treatment of a wide range of human diseases and it is essential that dental research, education, and practice keep pace with this rapidly advancing ?eld. As pointed out by Ford et al. (1): The de?nition of disease is also changing. Previously, disease was understood to be the presence of symptoms or of a particular phenotype. With increasing knowledge of the genetic basis of many diseases, this de?nition is changing to become the presence of a genotype conferring a pre-disposition to clinical symptoms or phenotype (Ford et al. (1)). This changing de?nition of disease means that today’s undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting. This naturally positions oral biology as an integral part of any dentally related professional’s repertoire of knowledge. There are as many topics in oral biology as there are the number of sites and micro- vironments within the oral cavity.

• Biofilm
• Bioinformatic analysis
• Dental diseases
• Dentistry
• Microarray
• Nanotechnology
• Oral cell biology
• Oral microbiota
• Saliva
• bioinformatics
• genome
• microbiology
• tissue

From the reviews:

“This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments … .” (Adam Roberts, Microbiology Today, May, 2011)

• Faculty of Dentistry, Department of Oral Sciences, University of Otago, Dunedin, New Zealand

  Gregory J. Seymour, Mary P. Cullinan

• Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand

  Nicholas C.K. Heng

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