Overview
- Editors:
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John R. Stephenson
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, Research & Development Division, Health Protection Agency, London, United Kingdom
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Alan Warnes
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Northwick Park Hospital, Research and Development, North West London Hospitals NHS Trust, Harrow, United Kingdom
- Highlights the major changes in the virus diagnostic laboratory
- Presents cutting-edge protocols in an easy to use, reproducible fashion with a focus on nucleic acid detection
- Includes expert notes on troubleshooting and avoiding common pitfalls
- Includes supplementary material: sn.pub/extras
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Table of contents (23 protocols)
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Front Matter
Pages i-xiii
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- Kanti Pabbaraju, Sallene Wong, Julie D. Fox
Pages 1-15
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- A. M. Powers, John T. Roehrig
Pages 17-38
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- Robert L. Atmar, Frederick H. Neill, Françoise S. Le Guyader
Pages 39-50
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- Nagesh K. Tripathi, Ambuj Shrivastava, Paban K. Dash, Asha M. Jana
Pages 51-64
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- Chit Laa Poh, Eng Lee Tan
Pages 65-77
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- Harald H. Kessler, Sophie Heyszl
Pages 101-121
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- Neil Berry, Carolina Herrera, Martin Cranage
Pages 133-160
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- Joanna S. Ellis, Martin D. Curran
Pages 161-181
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- Graham Tipples, Joanne Hiebert
Pages 183-193
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- Tarik Gheit, Massimo Tommasino
Pages 195-212
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- Andreas Kurth, Andreas Nitsche
Pages 257-278
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- Graeme Harkess, Anthony R. Fooks
Pages 279-307
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- Susan E. Sefers, Haijing Li, Yi-Wei Tang
Pages 309-323
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- Jim Gray, Miren Iturriza-Gómara
Pages 325-355
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- Sofi M. Ibrahim, Mohamed Aitichou, Justin Hardick, Jamie Blow, Monica L. O’Guinn, Connie Schmaljohn
Pages 357-368
About this book
Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.
Reviews
From the reviews of the second edition:
“In its 23 chapters it covers most … viruses of interest to the diagnostic virologist. Each chapter is written by an authority in the field and is composed of a short introduction followed by a number of protocols. These are sufficiently detailed to allow the reader to set up the assays in their own lab. … Most chapters address a virus type, but vary such that in some the introduction is very informative whilst in others it is merely a lead into the protocols.” (Dave Yirrell, Microbiology Today, November, 2011)
Editors and Affiliations
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, Research & Development Division, Health Protection Agency, London, United Kingdom
John R. Stephenson
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Northwick Park Hospital, Research and Development, North West London Hospitals NHS Trust, Harrow, United Kingdom
Alan Warnes
