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MicroRNAs and the Immune System

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Silvia Monticelli

    1. Institute for Research in Biomedicine, Bellinzona, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Offers cutting edge methods to analyse microRNA expression, function and post-transcriptional modifications
  • Includes methods to isolate and manipulate cells of the immune system
  • Provides a description of bioinformatics databases on miRNAs and their targets, as well as instructions to navigate them
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 667)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Analysis of miRNA Expression: Classic Methods Revisited

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Rapid, Quantitative Assay for Direct Detection of MicroRNAs and Other Small RNAs Using Splinted Ligation

      • Sangpen Chamnongpol, Patricia A. Maroney, Timothy W. Nilsen
      Pages 3-17

    3. Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs

      • Gary J. Latham
      Pages 19-31

    4. MicroRNA Detection in Bone Marrow Cells by LNA-FISH

      • Silvana Debernardi, Amanda Dixon-McIver
      Pages 33-45

    5. Measuring MicroRNA Expression in Size-Limited FACS-Sorted and Microdissected Samples

      • Kai P. Hoefig, Vigo Heissmeyer
      Pages 47-63

  3. High-Throughput Analysis of miRNAs

    1. Front Matter

      Pages 65-65
      Download chapter PDF

    2. MicroRNA Cloning from Cells of the Immune System

      • Haoquan Wu, Joel Neilson, N. Manjunath
      Pages 67-77

    3. High-Throughput Profiling in the Hematopoietic System

      • Muller Fabbri, Riccardo Spizzo, George A. Calin
      Pages 79-91

    4. Construction of Small RNA cDNA Libraries for Deep Sequencing

      • Molly F. Thomas, K. Mark Ansel
      Pages 93-111

    5. MicroRNA-Profiling in Formalin-Fixed Paraffin-Embedded Specimens

      • Ulrich Lehmann
      Pages 113-125

  4. Functional Analysis of miRNAs in the Immune System: Gain-of-Function

    1. Front Matter

      Pages 127-127
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    2. Expression of miRNAs in Lymphocytes: A Review

      • Raquel Malumbres, Izidore S. Lossos
      Pages 129-143

    3. Mouse Models for miRNA Expression: The ROSA26 Locus

      • Stefano Casola
      Pages 145-163

    4. Regulation of Monocytopoiesis by MicroRNAs

      • Laura Fontana, Antonio Sorrentino, Cesare Peschle
      Pages 165-176

    5. MicroRNA Activity in B Lymphocytes

      • Virginia G. de Yébenes, Almudena R. Ramiro
      Pages 177-192

    6. Isolation and Characterization of MicroRNAs of Human Mature Erythrocytes

      • Carolyn Sangokoya, Gregory LaMonte, Jen-Tsan Chi
      Pages 193-203

    7. Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors

      • Ramon J. Mayoral, Silvia Monticelli
      Pages 205-214

    8. Monitoring MicroRNA Activity and Validating MicroRNA Targets by Reporter-Based Approaches

      • Alessia Baccarini, Brian D. Brown
      Pages 215-233

  5. Functional Analysis of miRNAs in the Immune System: Loss-of-Function

    1. Front Matter

      Pages 235-235
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Keywords

About this book

In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.

Reviews

From the reviews:

“This book is useful to scientists seeking to study the role of miRNAs in the immune system. … helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. … The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students.” (Elizabeth S. Raveche, Doody’s Review Service, February, 2011)

“Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field … and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. … appealing to anyone from beginners to experts. … an attractive book for those interested in identifying and characterising particular miRNAs in the immune system.” (Daniel Peters, Immunology News, February, 2011)

Editors and Affiliations

  • Institute for Research in Biomedicine, Bellinzona, Switzerland

    Silvia Monticelli

Bibliographic Information

  • Book Title: MicroRNAs and the Immune System

  • Book Subtitle: Methods and Protocols

  • Editors: Silvia Monticelli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-811-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-810-2Published: 13 September 2010

  • Softcover ISBN: 978-1-4939-6150-4Published: 23 August 2016

  • eBook ISBN: 978-1-60761-811-9Published: 09 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 330

  • Topics: Immunology

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