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Tissue Microarrays

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Ronald Simon

    1. Institute of Pathology, University Medical Ctr Hamburg-Eppendorf, Hamburg, Germany

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  • Expert TMA protocols for all types of tissues including formalin fixed tissues, frozen tissues, and cell suspensions
  • Comprehensive description of in-situ techniques at the DNA, RNA, and protein level
  • Strategies for manual and automated TMA analysis
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 664)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Applications of Tissue Microarray Technology

    • Ronald Simon
    Pages 1-16

  3. Quality Aspects of TMA Analysis

    • Pierre Tennstedt, Guido Sauter
    Pages 17-26

  4. Representativity of TMA Studies

    • Guido Sauter
    Pages 27-35

  5. Recipient Block TMA Technique

    • Martina Mirlacher, Ronald Simon
    Pages 37-44

  6. Protocol for Constructing Tissue Arrays by Cutting Edge Matrix Assembly

    • Thai Hong Tran, Justin Lin, Ashley Brooke Sjolund, Fransiscus Eri Utama, Hallgeir Rui
    Pages 45-52

  7. Hypodermic Needle Without Recipient Paraffin Block Technique

    • Andréa Rodrigues Cordovil Pires, Simone Rabello de Souza
    Pages 53-61

  8. Resin Technologies: Construction and Staining of Resin TMA’s

    • William J. Howat, Susan J. Wilson
    Pages 63-72

  9. Tissue Microarrays from Frozen Tissues-OCT Technique

    • Marlena Schoenberg Fejzo, Dennis J. Slamon
    Pages 73-80

  10. An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples

    • Zhongting Hu, Elbert Chang, Melissa Hodeib
    Pages 81-91

  11. Building “Tissue” Microarrays from Suspension Cells

    • Shuchun Zhao, Yasodha Natkunam
    Pages 93-101

  12. Tissue Microarrays from Biopsy Specimens

    • Milton W. Datta, André A. Kajdacsy-Balla
    Pages 103-111

  13. Immunohistochemical Analysis of Tissue Microarrays

    • Ronald Simon, Martina Mirlacher, Guido Sauter
    Pages 113-126

  14. DNA Copy Number Analysis on Tissue Microarrays

    • Anne Kallioniemi
    Pages 127-134

  15. RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hybridization

    • Jürgen Veeck, Edgar Dahl
    Pages 135-150

  16. Automated Analysis of Tissue Microarrays

    • Marisa Dolled-Filhart, Mark Gustavson, Robert L. Camp, David L. Rimm, John L. Tonkinson, Jason Christiansen
    Pages 151-162

  17. Digital Microscopy for Boosting Database Integration and Analysis in TMA Studies

    • Tibor Krenacs, Levente Ficsor, Sebestyen Viktor Varga, Vivien Angeli, Bela Molnar
    Pages 163-175

  18. From Gene to Clinic: TMA-Based Clinical Validation of Molecular Markers in Prostate Cancer

    • Thorsten Schlomm, Felix KH Chun, Andreas Erbersdobler
    Pages 177-189

  19. Back Matter

    Pages 191-193
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Keywords

About this book

The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks. In this respect, the TMA invention was not frstly characterized by technical improvements, but its true novelty was to link clinical data to the tissues that were combined on one slide. The very high number of tissues that can be included into one TMA, the small size and regular shape of the tissue spots, the preser- tion of integrity of the donor tissue blocks, and the highly organized array pattern that allows for reliable allocation of clinical data to individual tissue spots made it a discrete technique with unique features. When the TMA technology was developed 12 years ago, its beneft was controversially debated. While many researchers welcomed the method enthusiastically, there were c- cerns by others that results obtained from the small tissue cores used for TMA making would not be suffciently representative of the donor tissues. Meanwhile, the increasing use of this technology has imposingly demonstrated its tremendous utility in research. In fact, basically all clinically relevant associations between molecular markers and clinical endpoints could be reproduced using only one single 0. 6 mm core per tissue sample so that TMAs have nowadays become a standard tool allowing for a new dimension of tissue analysis.

Reviews

From the reviews:

“This book is the first … to attempt to summarise the field of Tissue Microarrays (TMAs) since the first report of the technology in 1998. … relevant to the clinician-scientist who may be considering high-throughput analyses of genes and proteins from a large cohort. … In summary, this book is crammed with incredibly detailed technical advice … and even advice on whether to drill or cut the recipient paraffin blocks. … very readable, very informative and an excellent introduction to the gentle art of TMAs.” (Cris dos Remedios and Sean Lal, Proteomics, Vol. 11, 2011)

Editors and Affiliations

  • Institute of Pathology, University Medical Ctr Hamburg-Eppendorf, Hamburg, Germany

    Ronald Simon

Bibliographic Information

  • Book Title: Tissue Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Ronald Simon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-806-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-805-8Published: 19 August 2010

  • Softcover ISBN: 978-1-4939-6103-0Published: 23 August 2016

  • eBook ISBN: 978-1-60761-806-5Published: 09 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 193

  • Topics: Biochemistry, general

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