MAP Kinase Signaling Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Activation and Function of Components of the MAP Kinase Signaling Cascades

   1. Front Matter

      Pages 1-1

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   2. The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions

      • Yonat Keshet, Rony Seger

      Pages 3-38

   3. Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation

      • Shiri Procaccia, Sarah Kraus, Rony Seger

      Pages 39-58

   4. Activation of SAPK/JNKs In Vitro

      • Deborah N. Chadee, John M. Kyriakis

      Pages 59-73

   5. Activation of p38 and Determination of Its Activity

      • Huamin Zhou, Jianming Chen, Jiahuai Han

      Pages 75-89

   6. Activity Assays for Extracellular Signal-Regulated Kinase 5

      • Kazuhiro Nakamura, Gary L. Johnson

      Pages 91-106

   7. Use of Inhibitors in the Study of MAP Kinases

      • Kimberly Burkhard, Paul Shapiro

      Pages 107-122

3. Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals

   1. Front Matter

      Pages 123-123

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   2. MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration

      • Gabi Tarcic, Yosef Yarden

      Pages 125-135

   3. Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors

      • Mario Chiariello, Jose P. Vaqué, Piero Crespo, J. Silvio Gutkind

      Pages 137-150

   4. Regulation of MAP Kinase Signaling by Calcium

      • Colin D. White, David B. Sacks

      Pages 151-165

   5. Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates

      • Hui Zheng, Adnan Al-Ayoubi, Scott T. Eblen

      Pages 167-183

   6. ERK-MAP Kinase Signaling in the Cytoplasm

      • Michelle C. Mendoza, Ekrem Emrah Er, John Blenis

      Pages 185-203

   7. Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases

      • Marzia Indrigo, Alessandro Papale, Daniel Orellana, Riccardo Brambilla

      Pages 205-220

4. Structure-Function Relationships and Localization of MAP Kinases

   1. Front Matter

      Pages 221-221

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   2. Structural Studies of MAP Kinase Cascade Components

      • Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, Tianjun Zhou

      Pages 223-237

   3. Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry

      • Kevin M. Sours, Natalie G. Ahn

      Pages 239-255

   4. A “Molecular Evolution” Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases

      • Vered Levin-Salomon, Oded Livnah, David Engelberg

      Pages 257-272

   5. Reconstitution of the Nuclear Transport of the MAP Kinase ERK2

      • Arif Jivan, Aarati Ranganathan, Melanie H. Cobb

      Pages 273-285

Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases.

• Activation
• Cancer and inflammation
• Cell signaling
• Endoplasmatisches Reticulum
• Enzymatic activity
• Growth factors
• Immune System
• Immunity
• MAPK
• Migration
• Protein-protein interactions
• Regulation
• Subcellular localization
• Vivo
• cellular processes

• Dept. Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

  Rony Seger

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