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Immunotherapy of Cancer

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Patricia Yotnda

    1. , Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, USA

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  • Provides a wide range of detailed routine and state of the art techniques as well as experimental designs for cancer immunotherapy
  • Details protocols for the use of nanotechnology and stem cells in cancer immunotherapy
  • Covers pre-clinical models for cancer immunotherapy
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 651)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Hypoxic Tumors and Their Effect on Immune Cells and Cancer Therapy

    • Patricia Yotnda, Danli Wu, Anna May Swanson
    Pages 1-29

  3. Characterization of Regulatory T Cells in Tumor Suppressive Microenvironments

    • Guangyong Peng
    Pages 31-48

  4. Generation of Cytotoxic T Lymphocytes for Immunotherapy of EBV-Associated Malignancies

    • Corey Smith, Rajiv Khanna
    Pages 49-59

  5. Acquisition, Preparation, and Functional Assessment of Human NK Cells for Adoptive Immunotherapy

    • Dean A. Lee, Michael R. Verneris, Dario Campana
    Pages 61-77

  6. Enhanced Migration of Human Dendritic Cells Expressing Inducible CD40

    • Natalia Lapteva
    Pages 79-87

  7. Differentiation of Human Embryonic Stem Cells into Hematopoietic Cells In Vitro

    • Eun-Mi Kim, Nicholas Zavazava
    Pages 89-101

  8. Gene Therapy to Improve Migration of T Cells to the Tumor Site

    • Antonio Di Stasi, Biagio De Angelis, Barbara Savoldo
    Pages 103-118

  9. Gene Therapy to Improve Function of T Cells for Adoptive Immunotherapy

    • Concetta Quintarelli, Barbara Savoldo, Gianpietro Dotti
    Pages 119-130

  10. Cytokine-FC Fusion Genes as Molecular Adjuvants for DNA Vaccines

    • Daniel Hirschhorn-Cymerman, Miguel-Angel Perales
    Pages 131-155

  11. Pharmacology of Anti-CD3 Diphtheria Immunotoxin in CD3 Positive T-Cell Lymphoma Trials

    • Jung Hee Woo, Yu-Jen Lee, David M. Neville, Arthur E. Frankel
    Pages 157-175

  12. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display

    • Thomas Schirrmann, Michael Hust
    Pages 177-209

  13. Identification of Immunogenic Peptides of the Self-Tumor Antigen: Our Experience with Telomerase Reverse Transcriptase

    • Xochitl Cortez-Gonzalez, Maurizio Zanetti
    Pages 211-225

  14. Rescue, Amplification, Purification, and PEGylation of Replication Defective First-Generation Adenoviral Vectors

    • Michael A. Barry, Eric A. Weaver, Sean E. Hofherr
    Pages 227-239

  15. Adenovirus-Mediated Interleukin (IL)-24 Immunotherapy for Cancer

    • Rajagopal Ramesh, Constantine G. Ioannides, Jack A. Roth, Sunil Chada
    Pages 241-270

  16. Liposomes for Gene Transfer in Cancer Therapy

    • Nancy Smyth Templeton
    Pages 271-278

  17. Oncolytic HSV as a Vector in Cancer Immunotherapy

    • Hongtao Li, Xiaoliu Zhang
    Pages 279-290

  18. Generation of Chimeric T-Cell Receptor Transgenes and Their Efficient Transfer in Primary Mouse T Lymphocytes

    • Linda J. Howland, Nicole M. Haynes, Phillip K. Darcy
    Pages 291-306

  19. Multi-walled Carbon Nanotube (MWCNT) Synthesis, Preparation, Labeling, and Functionalization

    • Babak Kateb, Vicky Yamamoto, Darya Alizadeh, Leying Zhang, Harish M. Manohara, Michael J. Bronikowski et al.
    Pages 307-317

  20. Cellular Immunotherapy of Cancer

    • Fatma V. Okur, Malcolm K. Brenner
    Pages 319-345
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Keywords

About this book

A continuously evolving technique, immunotherapy for the treatment of cancers now incorporates the use of immune cells infused during bone marrow transplants as well as approaches like cell and gene therapy, while stem cell-based therapies, tissue engineering, and targeting have also contributed to the latest successes in pre-clinical immunotherapy studies. In Immunotherapy of Cancer: Methods and Protocols, expert researchers in the field describe detailed procedures for trainees and experts in the area of basic, clinical science who wish to undertake their own cutting-edge immunotherapy studies. In addition to the protocols, the volume also contains two general overviews providing useful updates in each area as well as summaries of recent pre-clinical and clinical trials. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Immunotherapy of Cancer: Methods and Protocols seeks to guide scientists along the path to a further developed system of immunotherapeutic treatments in order to reduce and hopefully eradicate this terrible disease.

Reviews

From the reviews:

“17 chapters devoted to techniques and their application to the treatment of cancer as well as two general overviews. … Both trainees and experts in basic and clinical science who wish to undertake studies involving immunotherapy are the intended audience. It would be useful for those who are not familiar with what immunotherapeutic procedures actually involve. … The book is well put together and offers a lot of information that would be helpful to someone about to embark on developing immunotherapeutic treatments for cancer.” (Marion C. Cohen, Doody’s Review Service, March, 2011)

Editors and Affiliations

  • , Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, USA

    Patricia Yotnda

Bibliographic Information

  • Book Title: Immunotherapy of Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Patricia Yotnda

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-786-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-785-3Published: 17 August 2010

  • Softcover ISBN: 978-1-4939-5753-8Published: 23 August 2016

  • eBook ISBN: 978-1-60761-786-0Published: 05 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 356

  • Topics: Immunology, Oncology, Antibodies

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