Skip to main content
SpringerLink
Log in
Book cover

RNAi and microRNA-Mediated Gene Regulation in Stem Cells

Methods, Protocols, and Applications

  • Book
  • © 2010

Overview

Editors:
  1. Baohong Zhang

    1. Dept. Biology, East Carolina University, Greenville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Edmund J. Stellwag

    1. Dept. Biology, East Carolina University, Greenville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes methods for state-of-the-art deep sequencing, miRNA-based shRNA-induced gene knockdown and qRT-PCR technologies
  • Protocols to identify and analyze the expression and function of RNAi and miRNA in stem cells
  • Authoritative guide to the experimental study of the nexus between RNAi and microRNA-mediated control of stem cell function
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 650)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. RNAi in Stem Cells: Current Status and Future Perspectives

      • Gang-Ming Zou
      Pages 3-14

    3. A Brief Introduction to RNAi and MicroRNAs in Stem Cells

      • Alexander K. Murashov
      Pages 15-25

  3. RNA Interference

    1. Front Matter

      Pages 27-27
      Download chapter PDF

    2. Forward RNAi Screens in Human Stem Cells

      • Christine Karlsson, Jonas Larsson, Aurélie Baudet
      Pages 29-43

    3. A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells

      • Melanie I. Trombly, Xiaozhong Wang
      Pages 45-63

    4. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells

      • Xiaoxing Cheng, Rui Jian
      Pages 65-74

    5. Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides

      • Stephen W. Chen, Steve K.W. Oh
      Pages 75-83

    6. Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector

      • Soizik Berlivet, Martin Houlard, Matthieu Gérard
      Pages 85-100

    7. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8

      • Takayuki Manabe, Akio Wanaka
      Pages 101-109

    8. Silencing of Rho-GDIγ by RNAi Promotes the Differentiation of Neural Stem Cells

      • Jiao Wang, Wei Lu, Tieqiao Wen
      Pages 111-120

    9. RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells

      • Gang-Ming Zou, Cynthia LeBron, Yumei Fu
      Pages 121-128

    10. Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and siRNA-Mediated Knock-Down

      • Carsten Hagemann, Harun M. Said, Michael Flentje, Klaus Roosen, Giles Hamilton Vince
      Pages 129-143

    11. An Efficient Transfection Method for Mouse Embryonic Stem Cells

      • Jun-Yang Liou, Bor-Sheng Ko, Tzu-Ching Chang
      Pages 145-153

    12. Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femtoinjection

      • Mikako Saito, Hideaki Matsuoka
      Pages 155-170

  4. MicroRNAs

    1. Front Matter

      Pages 171-171
      Download chapter PDF

    2. Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology

      • Ryan D. Morin, Yongjun Zhao, Anna-Liisa Prabhu, Noreen Dhalla, Helen McDonald, Pawan Pandoh et al.
      Pages 173-199

    3. Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary

      • Kin Chan, Hannele Ruohola-Baker
      Pages 201-212

    4. Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR)

      • Xiaoping Pan, Alexander K. Murashov, Edmund J. Stellwag, Baohong Zhang
      Pages 213-224
Back to top

Keywords

About this book

Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Editors and Affiliations

  • Dept. Biology, East Carolina University, Greenville, USA

    Baohong Zhang, Edmund J. Stellwag

Bibliographic Information

  • Book Title: RNAi and microRNA-Mediated Gene Regulation in Stem Cells

  • Book Subtitle: Methods, Protocols, and Applications

  • Editors: Baohong Zhang, Edmund J. Stellwag

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-769-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-768-6Published: 16 August 2010

  • Softcover ISBN: 978-1-4939-5749-1Published: 23 August 2016

  • eBook ISBN: 978-1-60761-769-3Published: 05 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 261

  • Topics: Cell Biology, Biochemistry, general, Human Genetics, Stem Cells, Gene Therapy

Publish with us

Policies and ethics

Back to top

Search

Navigation