Protein Misfolding and Cellular Stress in Disease and Aging

1. Front Matter

   Pages i-xi

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2. Concepts

   1. Front Matter

      Pages 1-1

      PDF

   2. Protein Misfolding and Cellular Stress: An Overview

      • Niels Gregersen, Peter Bross

      Pages 3-23

   3. Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance

      • Massimo Stefani

      Pages 25-41

   4. Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome

      • Martin Schröder, Louise Sutcliffe

      Pages 43-62

   5. What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases?

      • Alicia Mae Pickrell, Carlos Torres Moraes

      Pages 63-78

   6. Autophagy in Disease

      • Dalibor Mijaljica, Mark Prescott, Rodney J. Devenish

      Pages 79-92

   7. Mitophagy and Mitoptosis in Disease Processes

      • Dalibor Mijaljica, Mark Prescott, Rodney J. Devenish

      Pages 93-106

   8. Cellular Stress and Protein Misfolding During Aging

      • Rajiv Vaid Basaiawmoit, Suresh I. S. Rattan

      Pages 107-117

   9. Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques

      • Peter Bross, Johan Palmfeldt, Jakob Hansen, Søren Vang, Niels Gregersen

      Pages 119-135

3. Protocols

   1. Front Matter

      Pages 137-137

      PDF

   2. Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System

      • Kanako Wakabayashi-Nakao, Ai Tamura, Shoko Koshiba, Yu Toyoda, Hiroshi Nakagawa, Toshihisa Ishikawa

      Pages 139-159

   3. Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress

      • Christina Bak Pedersen, Niels Gregersen

      Pages 161-173

   4. Examining Ubiquitinated Protein Aggregates in Tissue Sections

      • Natalia A. Kaniuk, John H. Brumell

      Pages 175-182

   5. Determination of Proteasomal Activities

      • Cristovao F. Lima, Suresh I. S. Rattan

      Pages 183-192

   6. Measurement of Autophagy in Cells and Tissues

      • Isei Tanida, Satoshi Waguri

      Pages 193-214

   7. Transcription Factor Sequestration by Polyglutamine Proteins

      • Tomoyuki Yamanaka, Nobuyuki Nukina

      Pages 215-229

   8. Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity

      • Monica Bucciantini, Cristina Cecchi

      Pages 231-243

   9. Measurement of Mitochondrial ROS Production

      • Anatoly A. Starkov

      Pages 245-255

   10. In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria

       • Francesca Maltecca, Giorgio Casari

       Pages 257-267

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book’s coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.

• Disease processes
• Endoplasmatisches Reticulum
• Pathogenetic mechanisms
• Premature aging
• Termination
• Treatment strategies
• Vivo
• electron microscopy
• genes
• molecular biology
• proteins
• tissue
• transcription
• Proteomics

From the reviews:

“Methods in Molecular Biology deals with current topics and techniques in cellular stress as well as methods to study and analyze the effects of cellular stress on protein misfolding. … The book is written mainly for scientists involved in the study of diseases resulting from protein misfolding. … the protocol is straightforward and is based on proven examples that render these diverse methods credible. This should prove to be a valuable companion to all researchers in the field of cellular stress and protein misfolding.” (Ruben Mestril, Doody’s Review Service, February, 2011)

“Drs. Peter Bross e Niels Gregersen … invited scientists who are experts in Protein Misfolding to collaborate in the book’s edition … . focuses in the main points of the latest literature on Protein Misfolding on genesis of diseases and aging processes, and also provides experimental and analytical protocols employed to protein misfolding detection. … presented in two parts: the first one comprehends protein misfolding concepts, their importance on disease genesis, and the second one, describe experimental protocols employed in the investigation of intracellular alterations.” (Sandra Helena P. Farsky, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)

“This new volume constitutes an excellent opportunity to be driven on the causes and mechanisms that are actually know to produce the misfolding. … The concepts and protocols here presented are a good example of what the scientific community refers as molecular medicine: there is no need to change the academic curricula to teach the molecular bases of present day medicine, simply you have to well present on which physiological parameters the future doctor visit will sounds!” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

• Aarhus University Hospital, Research Unit for Molecular Medicine, University of Aarhus, Aarhus N, Denmark

  Peter Bross, Niels Gregersen

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