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Engineered Zinc Finger Proteins

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Joel P. Mackay

    1. School of Molecular &, Microbial Biosciences, University of Sydney, Sydney, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. David J. Segal

    1. Dept. Pharmacology & Genome Center, University of California, Davis, Davis, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Chapters from nearly all of the top research groups in the field
  • Sections on zinc finger nucleases, one of the most exciting tools for gene therapy and genome engineering in the past decade
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 649)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Engineering Zinc Finger Proteins

    1. Front Matter

      Pages 1-1
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    2. The Generation of Zinc Finger Proteins by Modular Assembly

      • Mital S. Bhakta, David J. Segal
      Pages 3-30

    3. Engineering Single Cys2His2 Zinc Finger Domains Using a Bacterial Cell-Based Two-Hybrid Selection System

      • Stacey Thibodeau-Beganny, Morgan L. Maeder, J. Keith Joung
      Pages 31-50

    4. Bipartite Selection of Zinc Fingers by Phage Display for Any 9-bp DNA Target Site

      • Jia-Ching Shieh
      Pages 51-76

    5. Structure-Based DNA-Binding Prediction and Design

      • Andreu Alibés, Luis Serrano, Alejandro D. Nadra
      Pages 77-88

  3. Artificial Transcription Factors

    1. Front Matter

      Pages 89-89
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    2. Generation of Cell-Permeable Artificial Zinc Finger Protein Variants

      • Takashi Sera
      Pages 91-96

    3. Inhibition of Viral Transcription Using Designed Zinc Finger Proteins

      • Kimberley A. Hoeksema, D. Lorne J. Tyrrell
      Pages 97-116

    4. Modulation of Gene Expression Using Zinc Finger-Based Artificial Transcription Factors

      • Sabine Stolzenburg, Alan Bilsland, W. Nicol Keith, Marianne G. Rots
      Pages 117-132

    5. Construction of Combinatorial Libraries that Encode Zinc Finger-Based Transcription Factors

      • Seokjoong Kim, Eun Ji Kim, Jin-Soo Kim
      Pages 133-147

    6. Silencing of Gene Expression by Targeted DNA Methylation: Concepts and Approaches

      • Renata Z. Jurkowska, Albert Jeltsch
      Pages 149-161

    7. Remodeling Genomes with Artificial Transcription Factors (ATFs)

      • Adriana S. Beltran, Pilar Blancafort
      Pages 163-182

    8. Transgenic Mice Expressing an Artificial Zinc Finger Regulator Targeting an Endogenous Gene

      • Claudio Passananti, Nicoletta Corbi, Annalisa Onori, Maria Grazia Di Certo, Elisabetta Mattei
      Pages 183-206

  4. Zinc Finger Nucleases

    1. Front Matter

      Pages 207-207
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    2. Artificial Zinc Finger Nucleases for DNA Cloning

      • Vardit Zeevi, Andriy Tovkach, Tzvi Tzfira
      Pages 209-225

    3. In Vitro Assessment of Zinc Finger Nuclease Activity

      • Toni Cathomen, Cem Şöllü
      Pages 227-235

    4. Quantification of Zinc Finger Nuclease-Associated Toxicity

      • Tatjana I. Cornu, Toni Cathomen
      Pages 237-245

    5. A Rapid and General Assay for Monitoring Endogenous Gene Modification

      • Dmitry Y. Guschin, Adam J. Waite, George E. Katibah, Jeffrey C. Miller, Michael C. Holmes, Edward J. Rebar
      Pages 247-256

    6. Engineered Zinc Finger Proteins for Manipulation of the Human Mitochondrial Genome

      • Michal Minczuk
      Pages 257-270
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Keywords

About this book

Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.

Editors and Affiliations

  • School of Molecular &, Microbial Biosciences, University of Sydney, Sydney, Australia

    Joel P. Mackay

  • Dept. Pharmacology & Genome Center, University of California, Davis, Davis, USA

    David J. Segal

Bibliographic Information

  • Book Title: Engineered Zinc Finger Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Joel P. Mackay, David J. Segal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-753-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-752-5Published: 06 August 2010

  • Softcover ISBN: 978-1-4939-5751-4Published: 23 August 2016

  • eBook ISBN: 978-1-60761-753-2Published: 03 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 500

  • Topics: Protein Science, Genetic Engineering

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