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Mass Spectrometry Imaging

Principles and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Stanislav S. Rubakhin

    1. , Beckman Institute, Univ. of Illinois at Urbana-Champaign, Urbana, USA

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  2. Jonathan V. Sweedler

    1. , Department of Chemistry, Univ. of Illinois at Urbana-Champaign, Urbana, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Illustrates mass spectrometry imaging on the sub-cellular, cellular, tissue, and whole body levels
  • Presents comprehensive information on sample preparation, data acquisition, and data processing approaches for use in bioanalytical mass spectrometry and mass spectrometry imaging
  • Includes a wide range of examples on the use of state-of-the-art mass spectrometric instrumentation
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 656)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Introductory Reviews and Tutorials

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Imaging Mass Spectrometry: Viewing the Future

      • Sarah A. Schwartz, Richard M. Caprioli
      Pages 3-19

    3. A Mass Spectrometry Primer for Mass Spectrometry Imaging

      • Stanislav S. Rubakhin, Jonathan V. Sweedler
      Pages 21-49

    4. Imaging of Metals, Metalloids, and Non-metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in Biological Tissues

      • J. Sabine Becker, J. Susanne Becker
      Pages 51-82

  3. Protocols for MS Imaging of Distribution of Small Molecules Including Metabolites and Pharmaceuticals

    1. Front Matter

      Pages 83-83
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    2. Lipid Detection, Identification, and Imaging Single Cells with SIMS

      • Michael L. Heien, Paul D. Piehowski, Nicholas Winograd, Andrew G. Ewing
      Pages 85-97

    3. The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids

      • Amina S. Woods, Shelley N. Jackson
      Pages 99-111

    4. Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Stores in Renal Epithelial Cells

      • Subhash Chandra
      Pages 113-130

    5. Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System

      • Yanfeng Chen, Ying Liu, Jeremy Allegood, Elaine Wang, Begoña Cachón-González, Timothy M. Cox et al.
      Pages 131-146

    6. Mapping Pharmaceuticals in Rat Brain Sections Using MALDI Imaging Mass Spectrometry

      • Yunsheng Hsieh, Fangbiao Li, Walter A. Korfmacher
      Pages 147-158

    7. Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry

      • Peter Nemes, Akos Vertes
      Pages 159-171

    8. Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol

      • Yuki Sugiura, Mitsutoshi Setou
      Pages 173-195

    9. Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS

      • A.F. Maarten Altelaar, Sander R. Piersma
      Pages 197-208

    10. Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue

      • Timothy J. Garrett, Richard A. Yost
      Pages 209-230

    11. Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry

      • Vilmos Kertesz, Gary J. Van Berkel
      Pages 231-241

    12. Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS)

      • Qiang Liu, Yongsheng Xiao, Lin He
      Pages 243-252

    13. Preparation of Single Cells for Imaging Mass Spectrometry

      • Elena S.F. Berman, Susan L. Fortson, Kristen S. Kulp
      Pages 253-265

    14. Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Types

      • Ligang Wu, James S. Felton, Kuang Jen J. Wu
      Pages 267-281

  4. Protocols for MS Imaging of Distribution of Peptides and Proteins

    1. Front Matter

      Pages 283-283
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Keywords

About this book

Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.

Reviews

From the reviews:

“The book aims to provide comprehensive background information on mass spectrometry imaging (MSI) technology … . a monograph that has the merit of summarizing many protocols of great value for all scientists, engineers, and clinicians who are interested in applying MSI in their work. The book is clearly written and comprehensive, and has a good balance between theoretical descriptions and broad practical descriptions. Both established MSI researchers and those new to the field would benefit from this valuable source of methodological information.” (José Manuel Costa-Fernández, Analytical and Bioanalytical Chemistry, Vol. 401, 2011)

Editors and Affiliations

  • , Beckman Institute, Univ. of Illinois at Urbana-Champaign, Urbana, USA

    Stanislav S. Rubakhin

  • , Department of Chemistry, Univ. of Illinois at Urbana-Champaign, Urbana, USA

    Jonathan V. Sweedler

Bibliographic Information

  • Book Title: Mass Spectrometry Imaging

  • Book Subtitle: Principles and Protocols

  • Editors: Stanislav S. Rubakhin, Jonathan V. Sweedler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-746-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-745-7Published: 06 August 2010

  • Softcover ISBN: 978-1-61779-696-8Published: 13 October 2012

  • eBook ISBN: 978-1-60761-746-4Published: 03 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 490

  • Topics: Mass Spectrometry, Imaging / Radiology, Spectroscopy/Spectrometry

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