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Cellular Programming and Reprogramming

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Sheng Ding

    1. The Scripps Research Institute, La Jolla, U.S.A.

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  • Provides authoritative protocols and insights in generating various essential cell types as written by leading researchers in the field
  • Details the process of producing functional cell types for high-throughput screens
  • Covers the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 636)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast

    • Ge Lin, Kristen Martins-Taylor, Ren-He Xu
    Pages 1-24

  3. Isolation and Maintenance of Mouse Epiblast Stem Cells

    • Josh G. Chenoweth, Paul J. Tesar
    Pages 25-44

  4. Functional Assays for Hematopoietic Stem Cell Self-Renewal

    • John M. Perry, Linheng Li
    Pages 45-54

  5. Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow

    • Kartik Subramanian, Martine Geraerts, Karen A. Pauwelyn, Yonsil Park, D. Jason Owens, Manja Muijtjens et al.
    Pages 55-78

  6. Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro

    • Yan Shi
    Pages 79-85

  7. Mesoderm Cell Development from ES Cells

    • Takumi Era
    Pages 87-103

  8. Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells

    • Shi-Jiang Lu, Qiang Feng, Jennifer S. Park, Robert Lanza
    Pages 105-121

  9. Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs

    • Bao-Yang Hu, Su-Chun Zhang
    Pages 123-137

  10. Directing Human Embryonic Stem Cells to a Retinal Fate

    • Thomas A. Reh, Deepak Lamba, Julianne Gust
    Pages 139-153

  11. Bovine Somatic Cell Nuclear Transfer

    • Pablo J. Ross, Jose B. Cibelli
    Pages 155-177

  12. Cell Fusion-Induced Reprogramming

    • Jeong Tae Do, Hans R. Schöler
    Pages 179-190

  13. An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells

    • Prashant Mali, Zhaohui Ye, Bin-Kuan Chou, Jonathan Yen, Linzhao Cheng
    Pages 191-205

  14. Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells

    • Caroline Desponts, Sheng Ding
    Pages 207-218

  15. Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression

    • Huafeng Xie, Catherine V. Laiosa, Thomas Graf
    Pages 219-232

  16. Reprogramming of B Cells

    • César Cobaleda
    Pages 233-250

  17. Adult Cell Fate Reprogramming: Converting Liver to Pancreas

    • Irit Meivar-Levy, Sarah Ferber
    Pages 251-283

  18. In Vitro Reprogramming of Pancreatic Cells to Hepatocytes

    • Daniel Eberhard, Kathy O’Neill, Zoë D. Burke, David Tosh
    Pages 285-292

  19. Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches

    • Wenlin Li, Sheng Ding
    Pages 293-300

  20. Small Molecule Screen in Zebrafish and HSC Expansion

    • Eirini Trompouki, Leonard I. Zon
    Pages 301-316
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Keywords

About this book

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body’s own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Editors and Affiliations

  • The Scripps Research Institute, La Jolla, U.S.A.

    Sheng Ding

Bibliographic Information

  • Book Title: Cellular Programming and Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Sheng Ding

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-691-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-690-0Published: 25 March 2010

  • Softcover ISBN: 978-1-4939-5714-9Published: 23 August 2016

  • eBook ISBN: 978-1-60761-691-7Published: 25 March 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 334

  • Topics: Biochemistry, general, Cell Biology, Stem Cells, Cell Culture

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