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Molecular and Cell Biology Methods for Fungi

  • Book
  • © 2010

Overview

Editors:
  1. Amir Sharon

    1. Dept. Plant Sciences, Tel Aviv University, Tel Aviv, Israel

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    You can also search for this editor in PubMed   Google Scholar

  • Provides a comprehensive set of methods used in fungal molecular and cell biology written by leading researchers in the field
  • Presents the most popular methods alongside highly advanced technologies
  • Methods cover all types of molecular and cellular research from single cell analyses to high throughput methods
  • Methods will prove invaluable to novice as well as experienced researchers
  • Methods have been proven effective for a wide variety of fungal species
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 638)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Fungal Transformation and Gene Knockout

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Protoplast Transformation of Filamentous Fungi

      • B. Gillian Turgeon, Bradford Condon, Jinyuan Liu, Ning Zhang
      Pages 3-19

    3. Electroporation and Agrobacterium-Mediated Spore Transformation

      • Anna Minz, Amir Sharon
      Pages 21-32

    4. High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains

      • Patrick D. Collopy, Hildur V. Colot, Gyungsoon Park, Carol Ringelberg, Christopher M. Crew, Katherine A. Borkovich et al.
      Pages 33-40

    5. Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi

      • Marie Dufresne, Marie-Josée Daboussi
      Pages 41-54

    6. DelsGate: A Robust and Rapid Method for Gene Deletion

      • María D. García-Pedrajas, Marina Nadal, Timothy Denny, Lourdes Baeza-Montañez, Zahi Paz, Scott E. Gold
      Pages 55-76

    7. Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression

      • Carmit Ziv, Oded Yarden
      Pages 77-100

  3. Detection and Quantification of Fungi

    1. Front Matter

      Pages 102-102
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    2. Analysis of Fungal Gene Expression by Real Time Quantitative PCR

      • Shahar Ish-Shalom, Amnon Lichter
      Pages 103-114

    3. Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization

      • Benjamin A. Horwitz, Sophie Lev
      Pages 115-123

    4. Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software

      • Paul H. Goodwin, Tom Hsiang
      Pages 125-135

    5. Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR

      • Pascale M. A. Seddas-Dozolme, Christine Arnould, Marie Tollot, Elena Kuznetsova, Vivienne Gianinazzi-Pearson
      Pages 137-152

    6. Application of Laser Microdissection to Study Plant–Fungal Pathogen Interactions

      • John Fosu-Nyarko, Michael G. K. Jones, Zhaohui Wang
      Pages 153-163

    7. Multiplex Gene Expression Analysis by TRAC in Fungal Cultures

      • Jari J. Rautio
      Pages 165-173

    8. Amplification of Fungal Genomes Using Multiple Displacement Amplification

      • Simon J. Foster, Brendon J. Monahan
      Pages 175-185
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Keywords

About this book

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. It is a diverse clade of heterotrophic eukaryotic organisms that shares some ch- acteristics with animals and includes mushrooms, molds, yeasts as well as many other types of less well known organisms. Approximately 100,000 species have been described, which comprise less than 10% of the estimated number of fungal species in nature. Fungi can be found in every place wherever adequate moisture, temperature, and organic substrates are available; however, they also occupy extreme habitats, from hot volcanoes to arctic zones, arid deserts, and deep oceans. The importance of fungi as a group is tremendous; most species are saprobes and play prime roles in decomposition and the recycling of organic matter and nutrients, and many of them produce enzymes and metabolites with important applications in pharmacology, biotechnology, and other industries. Alongside the positive aspects, fungi also cause huge damage, primarily as plant pathogens. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

Reviews

From the reviews:

“This volume of methods and step-by-step protocols for fungal molecular biology deals with three areas: (i) fungal transformation and gene knockout; (ii) detection and quantitation of fungi; and (iii) microscopy and protein analysis. The authors of the chapters are world leaders in their respective fields and the book … still a valuable laboratory resource. … this is a good place for a freshman fungal lab worker to start - and it also provides a useful list of contacts who no doubt could field further questions.” (Neil A. R. Gow, Microbiology Today, 2010)

Editors and Affiliations

  • Dept. Plant Sciences, Tel Aviv University, Tel Aviv, Israel

    Amir Sharon

Bibliographic Information

  • Book Title: Molecular and Cell Biology Methods for Fungi

  • Editors: Amir Sharon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-611-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-610-8Published: 18 March 2010

  • Softcover ISBN: 978-1-4939-6146-7Published: 23 August 2016

  • eBook ISBN: 978-1-60761-611-5Published: 18 March 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 321

  • Topics: Cell Biology, Biochemistry, general, Mycology, Eukaryotic Microbiology

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