Overview
- Provides a global overview of the state-of-the-art in RNA interference and practical details used experimentally in modern day experiments
- Deciphers, organizes, and explains the myriad of techniques used in gene silencing research
- Stimulates new experimental possibilities by gathering approaches that generally would not be present in the same volume
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 623)
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Table of contents (27 protocols)
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Front Matter
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Physiology of RNA Interference
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Front Matter
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RNA Interference in the Laboratory and siRNA Delivery
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Front Matter
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Keywords
About this book
From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and inspiring, RNA Interference: From Biology to Clinical Applications aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.
Reviews
From the reviews:
“The book provides a broad overview of RNA chemistry, the pharmacology and the manipulation of signaling pathways by using RNA interference. … This book is very well written and will be an asset to any researcher who uses RNA interference as a tool to study gene function. … and will probably mainly be of use to researcher already working in the field and familiar with different techniques of siRNA delivery.” (Anna Vossenkaemper, Immunology News, November, 2010)Editors and Affiliations
Bibliographic Information
Book Title: RNA Interference
Book Subtitle: From Biology to Clinical Applications
Editors: Wei-Ping Min, Thomas Ichim
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-588-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-587-3Published: 18 March 2010
Softcover ISBN: 978-1-4939-6134-4Published: 23 August 2016
eBook ISBN: 978-1-60761-588-0Published: 10 March 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 450
Topics: Immunology, Laboratory Medicine