Overview
- Provides a comprehensive overview of calcium imaging of the neural cells
- Presents a detailed account of state-of the art calcium indicators including fluorescent, luminescent, and genetically modified calcium probes
- Outlines wide array of techniques aimed at real-time calcium imaging of cellular organelles, subcellular compartments, individual cells, and neural networks
- Covers the theoretical and practical background of calcium indicators, as well as calcium imaging hardware and software
- Comprehensive guide to conceiving, developing, and running calcium imaging of nervous system
Part of the book series: Neuromethods (NM, volume 43)
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Table of contents (12 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.
Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.
Editors and Affiliations
Bibliographic Information
Book Title: Calcium Measurement Methods
Editors: Alexei Verkhratsky, Ole H. Petersen
Series Title: Neuromethods
DOI: https://doi.org/10.1007/978-1-60761-476-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-475-3Published: 17 December 2009
Softcover ISBN: 978-1-61779-677-7Published: 25 February 2012
eBook ISBN: 978-1-60761-476-0Published: 02 December 2009
Series ISSN: 0893-2336
Series E-ISSN: 1940-6045
Edition Number: 1
Number of Pages: XII, 256
Number of Illustrations: 51 b/w illustrations, 24 illustrations in colour
Topics: Neurosciences, Neurochemistry