Liposomes

1. Front Matter

   Pages i-xv

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2. Utilization of Liposomes for Studying Drug Transfer and Uptake

   • Alfred Fahr, Xiangli Liu

   Pages 1-10

3. The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles

   • James R. Reed

   Pages 11-20

4. Use of Liposomes to Study Cellular Osmosensors

   • Reinhard Krämer, Sascha Nicklisch, Vera Ott

   Pages 21-30

5. Studying Mechanosensitive Ion Channels Using Liposomes

   • Boris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny Petrov, Prithwish Pal, Alexander Fook Weng Foo et al.

   Pages 31-53

6. Studying Amino Acid Transport Using Liposomes

   • Cesare Indiveri

   Pages 55-68

7. Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes

   • Chris T. Höfer, Andreas Herrmann, Peter Müller

   Pages 69-82

8. Liposomal Reconstitution of Monotopic Integral Membrane Proteins

   • Zahra MirAfzali, David L. DeWitt

   Pages 83-94

9. The Reconstitution of Actin Polymerization on Liposomes

   • Mark Stamnes, Weidong Xu

   Pages 95-103

10. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions

    • L.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis A. Bagatolli et al.

    Pages 105-114

11. Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles

    • Martin Stöckl, Jörg Nikolaus, Andreas Herrmann

    Pages 115-126

12. Biosynthesis of Proteins Inside Liposomes

    • Pasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza, Pier Luigi Luisi

    Pages 127-145

13. Study of Respiratory Cytochromes in Liposomes

    • Iseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, Katia C. U. Mugnol

    Pages 147-165

14. Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity

    • Salette Reis, Marlene Lúcio, Marcela Segundo, José L. F. C. Lima

    Pages 167-188

15. Studying Colloidal Aggregation Using Liposomes

    • Juan Sabín, Gerardo Prieto, Félix Sarmiento

    Pages 189-198

16. Assessment of Liposome–Cell Interactions

    • Jan A. A. M. Kamps

    Pages 199-207

17. Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells

    • Nejat Düzgüneş, Henrique Faneca, Maria C. Pedroso de Lima

    Pages 209-232

18. The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes

    • David Miller, Paula J. Booth

    Pages 233-245

19. Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling

    • Witold K. Subczynski, Marija Raguz, Justyna Widomska

    Pages 247-269

20. Membrane Translocation Assayed by Fluorescence Spectroscopy

    • Jana Broecker, Sandro Keller

    Pages 271-289

Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.

• Biochemistry
• Biological membranes
• Biophysics
• Cholestrol
• FRET
• Glycolipids
• Lipids
• Phospholipids
• biosynthesis
• electron microscopy
• electron paramagnetic resonance spectroscopy
• enzymes
• fluorescence
• magnetic resonance spectroscopy
• metabolism

From the reviews:

“Provide a comprehensive guide to the preparation, characterisation, and potential use of currently known types of liposomes. They are written using an interdisciplinary approach, and so provide essential knowledge of liposomes, for not only researchers in this particular field, but also scientists in wider pharmaceutical and biological areas. … very inspiring – the potential for liposomes is immense – and these well-written chapters ignited notions of further applications – this is clearly a very exciting field.” (Trudy L. Knight, BTS Newsletter, Issue 37, Winter, 2010)

• College of Pharmacy, Midwestern University, Glendale, U.S.A.

  Volkmar Weissig

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