Overview
- Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types
- Explores diverse assays to measure DC function in the context of tolerance, autoimmune disease, allergy, and immunity to viral and bacterial infection
- All assays used currently by leading experts in the field for high impact studies
- Preluded by a digestible introduction to DC function and development, covering both humans and rodents
- Includes techniques that are cutting edge and state-of-the-art in the field of DC biology
- Contains a comprehensive list of models to test the effect that your favourite gene or immunological model has on DC development or function
- Helps readers to generate a particular specialized DC subtype in vitro saving time with greater focus on a biologically relevant system
- Examines isolating DC from almost all organs of the mouse
- Aids in the identification of the myriad subtypes in human and mice for further study
- Provides a standardized model for the use of human DCs in clinical trials
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 595)
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Table of contents (28 protocols)
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Front Matter
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Part I: Reviews
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Front Matter
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Part II: Human DC Protocols
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Front Matter
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Part III: Murine Dendritic Cell Methods
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Front Matter
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Keywords
About this book
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.
Reviews
From the reviews of the second edition:
“Written for principal investigators and their students, postdocs, and staff, providing very practical step-by-step instructions on how to work with DC. … Overall, this book provides a nice compilation of methods for studying dendritic cells. The book will be particularly useful for those studying murine DC, but will also provide some useful … information for those initiating human DC work. … this updated book, which is unique in the field, will make a nice addition to personal and institutional biomedical research libraries.” (Patricia Fitzgerald-Bocarsly, Doody’s Review Service, July, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Dendritic Cell Protocols
Editors: Shalin H. Naik
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-421-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-420-3Published: 17 December 2009
Softcover ISBN: 978-1-61779-676-0Published: 25 February 2012
eBook ISBN: 978-1-60761-421-0Published: 26 November 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVIII, 446
Number of Illustrations: 60 b/w illustrations, 6 illustrations in colour
Additional Information: Originally published as volume 64 in the series: Methods in Molecular Medicine
Topics: Immunology, Cell Biology, Laboratory Medicine