Overview
- Contains new total antioxidant capacity assays
- Includes colorimetric assays for hydroxyl radical and ethylene production analysis
- Explains the use of Laser Induced Breakdown Spectroscopy technique to analyze metals
- Provides oxidative hemolysis inhibition assay
- Examines the synthesis of alpha lipoic acid hybrids
- Explores the clinical use of pentaerythrityl tetranitrate as an antioxidant
- Delves into topics such as erythrocyte plasma membrane redox system, separation of thiols by HPLC-ECD, global index for determining oxidative balance, near patient testing, and in vivo pO2 using phosphorescence quenching
- Studies subjects like cellular chemiluminescence probe for redox state, 8-Isoprostane in breath condensate, oximetry and redoximetry using electron paramagnetic resonance spectroscopy, mitochondrial membrane potential, proton leak, and peroxide production
- Involves protocols on erythrocyte osmotic fragility as a biomarker for oxidative stress, high-throughput microplate assay for COX activity, effect of oxidative stress on insulin function, and redox imaging
- Delivers information on fluorescence-activated flow cytometry to measure oxidative stress
- Presents production and purification protocols for adenovirus vectors encoding antioxidant enzymes
- Offers examples of ChIP analysis for determining caveolin-1 gene transcription
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 594)
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Table of contents (30 protocols)
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Front Matter
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Reactive Oxygen and Nitrogen Techniques
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Front Matter
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Antioxidant Technology and Application
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Front Matter
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Keywords
About this book
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved.
Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.
Reviews
From the reviews:
“This is designed for use in graduate education and describes advanced protocols in oxidative stress in an easy to understand format. The book is intended for researchers, clinicians, graduate students, and junior researchers. I agree with the inclusion of clinicians, who will find this book valuable in determining a global assessment of redox status in their patients. … This volume provides a succinct account of protocols in oxidative stress and will be very useful for graduate students, clinicians, and junior and senior researchers.” (Omer Iqbal, Doody’s Review Service, June, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Advanced Protocols in Oxidative Stress II
Editors: Donald Armstrong
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-411-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-410-4Published: 07 December 2009
Softcover ISBN: 978-1-4939-5703-3Published: 23 August 2016
eBook ISBN: 978-1-60761-411-1Published: 14 January 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 444
Topics: Biochemistry, general, Cell Biology, Analytical Chemistry, Oxidative Stress