Overview
- Provides descriptions of existing heterologous expression systems for membrane proteins and instructions for manipulating these systems
- Contains methods for the purification of membrane proteins
- Includes information on classical detergents and alternatives for studying membrane proteins in solution
- Offers methods to study and increase membrane proteins stability
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 601)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
Reviews
From the reviews:
“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Heterologous Expression of Membrane Proteins
Book Subtitle: Methods and Protocols
Editors: Isabelle Mus-Veteau
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-344-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-343-5Published: 18 December 2009
Softcover ISBN: 978-1-4939-6132-0Published: 23 August 2016
eBook ISBN: 978-1-60761-344-2Published: 23 January 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 272
Topics: Protein Science, Biochemistry, general, Cell Biology, Proteomics, Membrane Biology