Overview
- Regarding Volume 1 and 2:
- Contains in-depth guides to isolation and processing of lipid samples, probes, lipid profiling, signaling, transport, and mapping
- Provides examples utilizing key model systems
- Presents oxidized lipids obtained from in vitro and in vivo biological material, redox platforms, high throughput systems, microscopy and imaging, and computer simulation
- Serves as a comprehensive guide to technical applications
- Explores bioinformatics strategy and statistical models for standardization of lipids
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 579)
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Table of contents (26 protocols)
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Front Matter
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Shotgun and Global Lipidomics
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Front Matter
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Analytical Approaches
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Front Matter
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Keywords
About this book
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
Editors and Affiliations
Bibliographic Information
Book Title: Lipidomics
Book Subtitle: Volume 1: Methods and Protocols
Editors: Donald Armstrong
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-322-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60761-321-3Published: 28 September 2009
Softcover ISBN: 978-1-61779-646-3Published: 09 August 2012
eBook ISBN: 978-1-60761-322-0Published: 18 September 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 550
Number of Illustrations: 139 b/w illustrations, 4 illustrations in colour
Topics: Lipidology, Biochemistry, general, Cell Biology, Membrane Biology