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Reverse Chemical Genetics

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Hisashi Koga

    1. Iwai Orthopaedic Medical Hospital, Tokyo, Japan

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  • Orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics
  • Introduces the fundamental resources and continues with the most recent molecular technologies
  • Presents applicable techniques and functional assays utilizing specific small molecules

Part of the book series: Methods in Molecular Biology (MIMB, volume 577)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics

    1. Front Matter

      Pages 1-1
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    2. ORFeome Cloning

      • Osamu Ohara
      Pages 3-9

    3. Systematic Cloning of an ORFeome Using the Gateway System

      • Akihisa Matsuyama, Minoru Yoshida
      Pages 11-24

    4. High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins

      • Hisashi Yamakawa
      Pages 25-39

    5. Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study

      • Makiko Suwa, Yukiteru Ono
      Pages 41-54

    6. High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data

      • Wataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada, Takeaki Taniguchi, Hideko Sone
      Pages 55-65

    7. Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology

      • T. S. Keshava Prasad, Kumaran Kandasamy, Akhilesh Pandey
      Pages 67-79

  3. Modification of cDNA Resources Utilized for Reverse Chemical Genetics

    1. Front Matter

      Pages 82-82
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    2. High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources

      • Kiyo Shimada, Hisashi Koga
      Pages 83-96

    3. An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources

      • Takashi Suzuki, Toru Ezure, Masaaki Ito, Masamitsu Shikata, Eiji Ando
      Pages 97-108

    4. Recombinant Protein Production by a Kaiko–Baculovirus System

      • Hidekazu Nagaya
      Pages 109-120

    5. Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins

      • Kei Yamaguchi, Shinichi Inoue, Osamu Ohara, Takahiro Nagase
      Pages 121-131

  4. The Refinement of Established Methods by Recent Innovations

    1. Front Matter

      Pages 134-134
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    2. Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine

      • Tadashi Kondo, Setsuo Hirohashi
      Pages 135-154

    3. Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis

      • Atsunori Hiratsuka, Kenji Yokoyama
      Pages 155-166

    4. Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos

      • Hirohito Shimizu, Kenta Uchibe, Hiroshi Asahara
      Pages 167-179

    5. Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins

      • Akito Tanaka
      Pages 181-195

  5. Potential Applicable Techniques for Reverse Chemical Genetics

    1. Front Matter

      Pages 198-198
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    2. Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases

      • Xiaobo Yu, Nicole Schneiderhan-Marra, Hsin-Yun Hsu, Jutta Bachmann, Thomas O. Joos
      Pages 199-214
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Keywords

About this book

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.

Editors and Affiliations

  • Iwai Orthopaedic Medical Hospital, Tokyo, Japan

    Hisashi Koga

Bibliographic Information

  • Book Title: Reverse Chemical Genetics

  • Book Subtitle: Methods and Protocols

  • Editors: Hisashi Koga

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-232-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60761-231-5Published: 24 September 2009

  • Softcover ISBN: 978-1-61779-641-8Published: 04 May 2012

  • eBook ISBN: 978-1-60761-232-2Published: 29 August 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 272

  • Number of Illustrations: 60 b/w illustrations, 4 illustrations in colour

  • Topics: Human Genetics, Gene Function

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