Overview
- Describes a breadth of uniquely suited model systems for analysis of chemotaxis, including bacteria, Dictyostelium, yeast, Drosophila, zebrafish, neurons, and immune and tumor cells
- Examines phenomena related to chemotaxis, including chemotropism, phototaxis, and electrotaxis
- Details the application of cutting-edge technologies for stimulating cells with chemoattractants and analyzing their resulting cellular behaviors
- Covers a variety of state-of-the-art microscopic methods for visualizing the migration of cells in tissues and the spatiotemporal dynamics of chemotaxis signaling molecules
- Provides step-by-step functional assays for a variety of components involved in chemotaxis, including cell-surface receptors, downstream effectors, and cytoskeletal components
- Explores the quantitative description of chemoattractant gradients and computational approaches for modeling the signaling networks that underlie gradient sensing
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 571)
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Table of contents (33 protocols)
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Front Matter
Keywords
About this book
Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.
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Editors and Affiliations
Bibliographic Information
Book Title: Chemotaxis
Book Subtitle: Methods and Protocols
Editors: Tian Jin, Dale Hereld
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-198-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60761-197-4Published: 25 September 2009
Softcover ISBN: 978-1-61779-642-5Published: 07 March 2012
eBook ISBN: 978-1-60761-198-1Published: 18 September 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 538
Number of Illustrations: 127 b/w illustrations, 5 illustrations in colour
Topics: Biochemistry, general, Cell Biology, Animal Biochemistry