Molecular Epidemiology of Microorganisms

1. Front Matter

   Pages i-xi

   PDF

2. Microbial Molecular Epidemiology: An Overview

   • Michel Tibayrenc

   Pages 1-12

3. Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens

   • Michel Tibayrenc

   Pages 13-25

4. Plasmid Replicon Typing

   • Timothy J. Johnson, Lisa K. Nolan

   Pages 27-35

5. The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms

   • Alex van Belkum, Elisabeth van Pelt-Verkuil, John P. Hays

   Pages 37-47

6. Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborne Pathogens

   • Kelli L. Hiett, Bruce S. Seal

   Pages 49-58

7. Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens

   • Tansy M. Peters

   Pages 59-70

8. Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance

   • David J. Ecker, Christian Massire, Lawrence B. Blyn, Steven A. Hofstadler, James C. Hannis, Mark W. Eshoo et al.

   Pages 71-87

9. Amplified Fragment Length Polymorphism Analysis

   • Norman K. Fry, Paul H.M. Savelkoul, Paolo Visca

   Pages 89-104

10. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens

    • Ibtissem Grissa, Gilles Vergnaud, Christine Pourcel

    Pages 105-116

11. Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex

    • Jeffrey R. Driscoll

    Pages 117-128

12. Multilocus Sequence Typing

    • Ana Belén Ibarz Pavón, Martin C.J. Maiden

    Pages 129-140

13. Multiple Locus Variable Number of Tandem Repeats Analysis

    • Gilles Vergnaud, Christine Pourcel

    Pages 141-158

14. Comparison of Molecular Typing Methods Applied to Clostridium difficile

    • Ed J. Kuijper, Renate J. van den Berg, Jon S. Brazier

    Pages 159-171

15. Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis

    • Pablo Bifani, Natalia Kurepina, Barun Mathema, Xiao-Ming Wang, Barry Kreiswirth

    Pages 173-188

16. spa Typing for Epidemiological Surveillance of Staphylococcus aureus

    • Marie Hallin, Alexander W. Friedrich, Marc J. Struelens

    Pages 189-202

17. Sequencing of Viral Genes

    • Carol Holm-Hansen, Kirsti Vainio

    Pages 203-215

18. Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterization of Foot-and-Mouth Disease Virus

    • Eleanor M. Cottam, Jemma Wadsworth, Nick J. Knowles, Donald P. King

    Pages 217-230

19. Bacterial Genome Sequencing

    • Hervé Tettelin, Tamara Feldblyum

    Pages 231-247

20. DNA Microarray for Molecular Epidemiology of Salmonella

    • Stephan Huehn, Burkhard Malorny

    Pages 249-285

The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains. A wide range of techniques has been applied to the investigation of outbreaks of tra- missible disease, and these have been critical in unraveling the route of spread of pat- gens for humans, animals, and plants. The choice of a molecular method will depend on the type of questions to be addressed, on the degree of genetic diversity of the species to be analyzed, and on the mechanisms responsible for generation of the diversity. The applications of molecular methods, singly or in combination, have greatly contributed in the past two decades to basic microbial science and public health control strategies. Molecular Epidemiology of Microorganisms: Methods and Protocols brings together a series of methods-based chapters with examples of application to some of the most important microbes. Both traditional and novel techniques are described, and the type of information that can be expected to be obtained by their application is indicated.

• Bacterial genome sequencing
• Epidemiological
• Epidemiology
• Multilocus sequence typing
• Multiple locus VNTR
• Pulsed-field gel electrophoresis
• Randomly amplified DNA analysis
• Rep-PCR
• Spa-typing
• Viral gene sequencing
• molecular epidemiology

From the reviews:

"This book provides valuable insight into the molecular genotyping methods for differentiation and classification of microorganisms at the subspecies level … . It is intended for researchers in the field of molecular epidemiology of microorganisms. Students as well as experts in the field will find this book quite informative and useful. … This is a valuable resource providing state-of-the-art techniques applied to the investigation of transmissible diseases, enabling researchers to unravel the routes of spread of pathogens for humans, animals, and plants." (Omer Iqbal, Doody’s Review Service, September, 2009)

Policies and ethics