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Neural Cell Transplantation

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. David Gordon
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    You can also search for this editor in PubMed   Google Scholar

  2. Neil J. Scolding
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    You can also search for this editor in PubMed   Google Scholar

  • Provides concise introductory overviews providing a comprehensive background to the field
  • Includes bench-to-bedside coverage spanning the whole spectrum of practical procedures from laboratory to clinic
  • Written throughout by leading, active researchers in the field
  • Describes detailed methodologies reproducible at the bench
  • A chapter per technique, with no further reference-chasing

Part of the book series: Methods in Molecular Biology (MIMB, volume 549)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introductory Reviews

    1. Front Matter

      Pages 1-1
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    2. Neural Transplantation and Stem Cells

      • Mahendra S. Rao, Mohan C. Vemuri
      Pages 3-16

    3. Adult Stem Cells for the Treatment of Neurological Disease

      • C.M. Rice, N.J. Scolding
      Pages 17-32

    4. Human Trials for Neurodegenerative Disease

      • Claire M. Kelly, O.J. Handley, A.E. Rosser
      Pages 33-47

  3. Practical/Methodological Chapters

    1. Front Matter

      Pages 50-50
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    2. Differentiation of Neuroepithelia from Human Embryonic Stem Cells

      • Xiaofeng Xia, Su-Chun Zhang
      Pages 51-58

    3. Derivation of High-Purity Oligodendroglial Progenitors

      • Maya N. Hatch, Gabriel Nistor, Hans S. Keirstead
      Pages 59-75

    4. Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny

      • Preethi Eldi, Rodney L. Rietze
      Pages 77-89

    5. Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay

      • Loic P. Deleyrolle, Brent A. Reynolds
      Pages 91-101

    6. Human Mesenchymal Stem Cell Culture for Neural Transplantation

      • David Gordon, Neil J. Scolding
      Pages 103-118

    7. Umbilical Cord Blood Cells

      • Jennifer D. Newcomb, Alison E. Willing, Paul R. Sanberg
      Pages 119-136

    8. Animal Models of Neurodegenerative Diseases

      • Wendy Phillips, Andrew Michell, Harald Pruess, Roger A. Barker
      Pages 137-155

    9. Animal Models of Multiple Sclerosis

      • Roberto Furlan, Carmela Cuomo, Gianvito Martino
      Pages 157-173

    10. Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies

      • Yoichi Kondo, Ian D. Duncan
      Pages 175-185

    11. A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery

      • Masanori Sasaki, Osamu Honmou, Jeffery D. Kocsis
      Pages 187-195

    12. MR Tracking of Stem Cells in Living Recipients

      • Eva Syková, Pavla Jendelová, Vít Herynek
      Pages 197-215

    13. Identifying Neural Progenitor Cells in the Adult Brain

      • Stephen Kelly, Maeve Caldwell, Matthew P. Keasey, Jessica A. Cooke, James B. Uney
      Pages 217-230

    14. Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of Poor Prognosis Multiple Sclerosis

      • Harold Atkins, Mark Freedman
      Pages 231-246

  4. Back Matter

    Pages 247-253
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Keywords

About this book

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

Reviews

From the reviews: “In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. … Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. … the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs … . a good book for neuroscientists who are directly involved in the separation and production of human stem cells.” (Celso Agner, Doody’s Review Service, December, 2009)

Bibliographic Information

  • Book Title: Neural Cell Transplantation

  • Book Subtitle: Methods and Protocols

  • Editors: David Gordon, Neil J. Scolding

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-931-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-930-7Published: 07 May 2009

  • Softcover ISBN: 978-1-61737-919-2Published: 19 November 2010

  • eBook ISBN: 978-1-60327-931-4Published: 20 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 226

  • Number of Illustrations: 32 b/w illustrations

  • Topics: Cell Biology, Stem Cells

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