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Bacteriophages

Methods and Protocols, Volume 2: Molecular and Applied Aspects

  • Book
  • © 2009

Overview

Editors:
  1. Martha R.J. Clokie

    1. University of Leicester, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrew M. Kropinski

    1. Public Health Agency of Canada, Guelph, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Examines tried-and-true methods along with the most cutting-edge protocols
  • Ideal for both professionals and newcomers due to convenient, easy-to-use style
  • Designed for modular use, providing a potential wide array of techniques based on the combination of the methods described

Part of the book series: Methods in Molecular Biology (MIMB, volume 502)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xxii
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  2. Bacteriophage Genomics

    1. Front Matter

      Pages 1-1
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    2. Preparation of Bacteriophage Lysates and Pure DNA

      • Derek John Juan Pickard
      Pages 3-9

    3. Approaches to the Compositional Analysis of DNA

      • Richard A. Manderville, Andrew M. Kropinski
      Pages 11-17

    4. Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis

      • Erika Lingohr, Shelley Frost, Roger P. Johnson
      Pages 19-25

    5. Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing

      • Elizabeth J. Summer
      Pages 27-46

    6. PCR and Partial Sequencing of Bacteriophage Genomes

      • Martha Clokie
      Pages 47-55

    7. In Sffamily Identification of Genes in Bacteriophage DNA

      • Andrew M. Kropinski, Mark Borodovsky, Tim J. Carver, Ana M. Cerdeño-Tárraga, Aaron Darling, Alexandre Lomsadze et al.
      Pages 57-89

    8. Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions

      • Sherwood R. Casjens, Eddie B. Gilcrease
      Pages 91-111

    9. In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators

      • Rob Lavigne, André Villegas, Andrew M. Kropinksi
      Pages 113-129

    10. Molecular Phylogenetics: Testing Evolutionary Hypotheses

      • David A. Walsh, Adrian K. Sharma
      Pages 131-168

  4. Community Bacteriophage Approaches

    1. Front Matter

      Pages 253-253
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    2. Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinting Methods

      • Clemens Pausz, Jessica L. Clasen, Curtis A. Suttle
      Pages 255-278

    3. Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome

      • K. Eric Wommack, Shellie R. Bench, Jaysheel Bhavsar, David Mead, Tom Hanson
      Pages 279-289
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Keywords

About this book

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Editors and Affiliations

  • University of Leicester, UK

    Martha R.J. Clokie

  • Public Health Agency of Canada, Guelph, Canada

    Andrew M. Kropinski

Bibliographic Information

  • Book Title: Bacteriophages

  • Book Subtitle: Methods and Protocols, Volume 2: Molecular and Applied Aspects

  • Editors: Martha R.J. Clokie, Andrew M. Kropinski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-565-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-564-4Published: 15 December 2008

  • Softcover ISBN: 978-1-61737-910-9Published: 19 November 2010

  • eBook ISBN: 978-1-60327-565-1Published: 11 December 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 373

  • Topics: Bacteriology, Microbiology, Laboratory Medicine

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