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siRNA and miRNA Gene Silencing

From Bench to Bedside

  • Book
  • © 2009

Overview

Editors:
  1. Mouldy Sioud

    1. Institute for Cancer Research, Dept. Immunology, Norwegian Radium Hospital, Montebello, Oslo, Norway

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  • Unparalleled diversity of siRNA delivery and imaging strategies covered in a single volume
  • Details the strategies that predict the design of effective siRNA with minimum side effects
  • Fully explains miRNA biogenesis and how to design microRNA-based vectors and to apply them in vivo
  • Explores the clinical potential of siRNA in human diseases, and describes the first application in patients with cancer

Part of the book series: Methods in Molecular Biology (MIMB, volume 487)

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Table of contents (22 protocols)

  1. Front Matter

    Pages 1-13
    Download chapter PDF

  2. Methods for Selecting Effective siRNA Sequences by using Statistical and Clustering Techniques

    • Shigeru Takasaki
    Pages 1-39

  3. Deciphering the Code of Innate Immunity Recognition of siRNAs

    • Mouldy Sioud
    Pages 1-19

  4. Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells

    • Mouldy Sioud
    Pages 1-22

  5. Local and Systemic Delivery of siRNAs for Oligonucleotides Therapy

    • Fumitaka Takeshita , Naomi Hokaiwado , Kimi Honma , Agnieszka Banas , Takahiro Ochiya
    Pages 1-10

  6. Imaging of siRNA Delivery and Silencing

    • Anna Moore , Zdravka Medarova
    Pages 1-18

  7. Recent Advances in Magnetofection and Its Potential to Deliver siRNAs In Vitro

    • Olga Mykhaylyk , Olivier Zelphati , Edelburga Hammerschmid , Martina Anton , Joseph Rosenecker , Christian Plank
    Pages 1-36

  8. In Vitro and In Vivo Gene Silencing by TransKingdom RNAi (tkRNAi)

    • Shuanglin Xiang , Andrew C. Keates , Johannes Fruehauf , Youxin Yang , Hongnian Guo , Thu Nguyen et al.
    Pages 1-14

  9. Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy

    • De-Qi Xu , Ling Zhang , Dennis J Kopecko , Lifang Gao , Yueting Shao , Baofeng Guo et al.
    Pages 1-27

  10. The Therapeutic Potential of LNA-modified siRNAs: Reduction of Off-target Effects by Chemical Modification of the siRNA Sequence

    • Kees Fluiter , Olaf R. F. Mook , Frank Baas
    Pages 1-15

  11. pSM155 and pSM30 Vectors for miRNA and shRNA Expression

    • Junzhu Wu , Akua N. Bonsra , Guangwei Du
    Pages 1-15

  12. Targeting Oncogenes with siRNAs

    • Olaf Heidenreich
    Pages 1-22

  13. Targeting Stromal-cancer Cell Interactions with siRNAs

    • Seyedhossein Aharinejad, Mouldy Sioud, Trevor Lucas, Dietmar Abraham
    Pages 1-24

  14. Therapeutic Potential of siRNA-mediated Targeting of Urokinase Plasminogen Activator, Its Receptor, and Matrix Metalloproteinases

    • Christopher S. Gondi, Jasti S. Rao
    Pages 1-15

  15. Silencing [AU1] of HIF-1α by RNA Interference in Human Glioma Cells In Vitro and In Vivo

    • David L. Gillespie, Jeannette R. Flynn, Brian T. Ragel, Maria Arce-Larreta, David A. Kelly, Sheryl R. Tripp et al.
    Pages 1-19

  16. RNA Interference-Mediated Validation of Genes Involved in Telomere Maintenance and Evasion of Apoptosis as Cancer Therapeutic Targets

    • Marco Folini, Marzia Pennati, Nadia Zaffaroni
    Pages 1-28

  17. Treating Respiratory Viral Diseases with Chemically Modified, Second Generation Intranasal siRNAs

    • Sailen Barik
    Pages 1-11

  18. Progress in the Therapeutic Applications of siRNAs Against HIV-1

    • Miguel Angel Martínez
    Pages 1-26

  19. Protein Components of the microRNA Pathway and Human Diseases

    • Marjorie P. Perron, Patrick Provost
    Pages 1-17

  20. Intron-Mediated RNA Interference and microRNA Biogenesis

    • Shao-Yao Ying, Shi-Lung Lin
    Pages 1-27
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Keywords

About this book

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection.

Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

Editors and Affiliations

  • Institute for Cancer Research, Dept. Immunology, Norwegian Radium Hospital, Montebello, Oslo, Norway

    Mouldy Sioud

Bibliographic Information

  • Book Title: siRNA and miRNA Gene Silencing

  • Book Subtitle: From Bench to Bedside

  • Editors: Mouldy Sioud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-547-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-546-0Published: 26 November 2008

  • Softcover ISBN: 978-1-61737-908-6Published: 26 May 2011

  • eBook ISBN: 978-1-60327-547-7Published: 03 July 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 472

  • Topics: Biochemistry, general, Human Genetics, Gene Expression

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